Objective To explore the production procedure of highly purified Mycobacterium bovis BCG genome DNA (BCG-DNA). Methods Disrupt BCG by ultrasonication and denaturalize the somatic protein by heating.

• 目的 摸索制备高纯度卡介菌 (MycobacteriumbovisBacillusCalmette Gu??in ,BCG)基因组DNA(BCG -DNA)的制备工艺。