Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin, and confirmed by GUS histochemical staining, polymerase chain reaction (PCR), and southern blot analysis.

  • 器官发生的转基因愈伤组织和转基因的再生植株已经获得 ,并经GUS组织化学染色、聚合酶链式反应和Southern杂交分析证实。
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