Papain was preliminarily purificated by Hitrap SP column with FPLC system, and the enzyme activity was estimated by Kimmel &Smith method.

• 应用FPLC系统对采自云南峨山县的番木瓜乳汁中的木瓜蛋白酶进行了初步纯化,同时,用基梅尔-史密斯法测定了番木瓜乳汁中木瓜蛋白酶的活性。