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- 合成的方案是采用先合成AGD、CGG和DAG三个三核苷二磷酸片段,然后再以AGD+pCGGDAG(3+p6)或AGDCGG+pDAG(6+p3)两种方式用T_4 RNA连接酶连接成为所需要的九核苷酸。As the first step, three trinucleoside diphosphate fragments AGD, CGG and DAG were synthesized and then, in the presence of T_4 RNA ligase, joined to form the desired nonamer either through route 3+ p6 (AGD+ pCGGDAG) or route 6+p3 (AGDCGG+pDAG). The phosphodiester methed was used in the syntheses of CGG and DAG;
- 泛素连接酶ubiquitin ligase, ubiquitinligating enzyme
- 辅酶A连接酶CoA ligase
- 酶的enzymatic
- 泛素连接酶E3Ubiquitin ligase E3
- 辅酶coenzyme
- 酶解zymohydrolysis
- 过氧化氢酶catalase
- 纤维素酶cellulase
- 碳氮连接酶类Carbon-nitrogen ligases
- 酶活性enzyme activity
- 酶学enzymology
- 碱性磷酸酶alkaline phosphatase
- E3泛素连接酶E3 ligase
- 连接酶合成产物的瞬时速度与细胞中可利用的ATP含量成比例。The degree of bond formation by ligases is proportional to the amount of ATP available in the cell at a particular instant.
- 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
- 氨基酸RNA连接酶amino acid REN ligase
- 氨基酸核糖核酸连接酶amino-acid-RNA ligase
- 醛连接酶carboligase
- DNA连接酶DNA ligase; DNA sealase; polydeoxyribonucleotide synthase
