Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment.

• 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。