Shim-pack VP-ODS is used as column, ACN: EtOAc(21:3, V/V) as mobile B, and aqueous acetic acid (50:1,V/V) as mobile A, linear gradient elution is adopted.

• 流动相A为2%25醋酸(HAc)，流动相B为乙腈(ACN)； 乙酸乙酯(EtOAc)=21：3(v/v)，梯度洗脱；