Some cells were loaded with Fluo-3/AM, and the dynamic change of [Ca2+]i in single pancreatic acinar cell was determined by laser scanning confocal microscopy.

• 部分胰腺腺泡细胞经Fluo-3/AM负载后,于相应的时点采用灌流方式给予药物或刺激剂,激光共聚焦显微镜观察单个胰腺腺泡细胞[Ca2+]i的变化。