The karyotes were separated by density gradient centrifugation, and the stroma cells of the bone marrow were gained adherent separation method.

• 选择雄性6周龄SD大鼠4只,抽取大鼠股骨骨髓,使用密度梯度离心分离有核细胞,贴壁分离法获取骨髓基质细胞,以2×104/cm2的密度接种进行细胞传代与分组实验。