The purification of kallikrein was improved by means of macro-porous anion-exchange resin.The purified product had specific activity 6 timeshigher than the crude one.The purification recovery was 90%.

• 应用国产大孔型强碱性阴离子交换树脂纯化激肽释放酶,精制回收率为90%25,精品平均比活力比粗品提高6倍,比有机溶剂沉淀法为优。