The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.

• 摘要 本研究利用聚合酶链式反应技术,成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。