The reproducibility, selectivity and lifetime of tissue membrane electrode were determinated, The Michaelis constant of enzyme reaction at present condition was calculated.

• 研究了介质条件、温度、切片厚度、固定化等条件的影响,测定了电极的重现性、选择性和土豆切片的活性期限,计算了该条件下酶反应的Michaelis常数。