Then the tissue engineering bone graft was enveloped by the rabit sacrospinal muscles. On the 4th,8th and 12th week after operation,the grafts were harvested and taken to Masson trichrome stain.

• 将人工骨移植到兔骶棘肌肌袋内,于术后4、8、12周进行Masson三色法组织学观察、扫描及透射电镜观察,观察其骨化及血管化情况。