This method is advisable. The purified protein has a molecular mass of 10.91 kDa as analyzed by SDS-PAGE, which is similar to the report of Cavanagh AC.

• 这条分离纯化早孕因子的技术路线是可行的；得到的相对分子量为10.；91kDa的蛋白质为电泳纯的早孕因子。