Trafficking of Mac-1-FP was successfully traced by watching blue fluorescence from fusion proteins CD11b-BFP and yellow fluorescence from YFP-CD18 when excited by 380 and 510nm respectively.
英
美
- 24小时PMA再次作用,部分CD11b与CD18可再次出现在膜上,说明Mac-1还可以被循环利用; (3)PMA刺激后Mac-1-FP由胞浆内转位到质膜上然后内吞的这一动态过程,与PMA活化后流式细胞术测得膜上Mac-1-FP以及粘附活性的变化完全符合;