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- Gelfiltration negative anion exchange column chromatography with high performance liguid chromatography system and plant hemagglutinin affinity chromatography were used for the isolation and purification of blood group substances from saliva. 方法:应用凝胶过滤、阴离子高速液相色谱及PHA亲合层析法,从人唾液中分离纯化血型物质。
- Galangin was obtained by on-step column chromatography of polyamide only once. 运用聚酰胺柱层析-次分离,获得有效成分高良姜素。
- Its homogeneity was identified with Sepharose 6B column chromatography. 紫外光谱显示它不含蛋白质、多肽及核酸。
- Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS|PAGE and stained using Con A|HRP. 用亲和层析,SDS?PAGE 及印迹技术,从玉米精细胞中分离纯化质膜蛋白。
- In this paper, we extracted the antioxidative substance from Alpinia oxyphylla Miquel by using column chromatography. 摘要探讨了干柱层析法分离益智渣及益智乙醇提取物的抗氧化成分。
- METHODS: Alcoholic extraction, silica gel, ODS column chromatography and recrystallization were used. 方法:采用工业酒精提取、胶柱层析、DS柱层析和重结晶等方法。
- MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. 方法 AchE单克隆抗体是一种IgG1抗体 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA亲和层析柱进行提取纯化 ,获得纯化的单克隆抗体Fab段。
- METHODS CTX-d was fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC. 方法应用柱层析及RP-HPLC,从眼镜蛇毒粗毒中分离纯化细胞素素(CTX)。
- Results Galangin was obtained by on-step column chromatography of polyamide only once. 结果运用聚酰胺柱层析-次分离,获得有效成分高良姜素。
- Objective To separate flavonol and flavanonol by the column chromatography of polyamide. 目的采用聚酰胺柱色谱分离黄酮醇与二氢黄酮醇类化合物。
- To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium. 目的建立葡聚糖凝胶色谱法测定头孢替坦二钠中聚合物的方法。
- Results Pure flavonol and flavanonol were obtained by the column chromatography of polyamide. 结果运用聚酰胺柱色谱法一次分离得高纯度黄酮醇与二氢黄酮醇。
- The molecular weight of the antibiotic substance is 48000 Dalton,tested by sephadex-G75 gel column chromatography. 而且用Sephadex-G75凝胶柱层析法测得抗生物质的分子量为48000道尔顿
- Conclusion: Silica gel column chromatography and crystallization are simple and rapid methods to isolate gardenoside. 结论:柱层析和重结晶方法是简单、速分离栀子苷的有效办法。
- The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis. 以这些亲生物性溶胶管柱来作样品清理的应用潜力,已由环境、食物及医疗上获得证明。
- The extract was further purified by column chromatography to get the acicular crystals of reserpine of 99. 将萃取所得产物用硅胶柱色谱进一步分离提纯;得到了质量分数为99.;8%25的利血平针状结晶。
- Methods The compounds were isolated by silica gel column chromatography and identified by spec-tral analysis. 方法通过硅胶柱色谱法分离纯化化合物,谱学方法鉴定其结构。
- Then the extracted material was sequently filtered and sperated by means of silica gel column chromatography. 提取物通过硅胶柱层析分离,得到无辣味的红色色素。
- Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. 目的:对东北特产平贝母的生物碱进行分离纯化研究。
- Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 结果3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%25左右,Fab抗体柱纯化的Fab抗体的纯度为95%25,但这两种亲和柱的目的蛋白收率都不高,分别为35%25、55%25。