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Insert and ligate any DNA fragment into a vector such as plasmid or viral vector, to form a recombinant DNA. 通常重组DNA一词指的是将任何细胞来源的DNA片段接在染色体外的能迅速复制的质粒或病毒载体。
Vector retargeting is one of the important strategies on viral vector targeting. 载体的靶向策略很多,载体重靶向就是其中重要的一种。
AIM:To study the safety of intramuscular injection of non viral vector pNMG3 of recombinant human growth hormone gene(hGH). 目的:研究携带重组人生长激素(hGH)基因非病毒载体pNMG3骨骼肌注射方法的急性期安全性。
The different of the two plasmid lies in different Promotors,since a strong promotor may paly a important role in gene expression in viral vector. 他们的区别在于含有不同的启动子，因为强启动子可能在病毒载体系统的基因表达上起关键的作用。
Gene therapy researchers have found that a popular viral vector used to * disease-curing genes into mice has a dangerous down side: it can apparently trigger liver tumors. 基因治疗的研究者们已经发现一个常用来插入治病基因的病毒载体有危险的副作用：明显地，它能够导致肝癌的发生。
HCN genes have been delivered to animal myocardium via viral vectors or HCN-transferred cells for recreating biological pacemakers. 利用病毒载体或转染HCN基因的细胞将HCN基因导入动物心脏内可重建生物起搏点。
Viral Vectors for Gene Therapy-Methods and Protocols. 病毒载体在基因治疗中的应用。
Animal quarantine: major animal epidemics, poultry diseases and aquatic animals viral diseases identification; fur products. 动物检疫:大动物疫病、禽类疫病及水生动物病毒性疾病的检疫鉴定,皮毛及制品中炭疽芽孢菌的检测。
Thus, overexpression of the glucose transporter gene via the introduction of a herpes simplex viral vector into the nervous system protected neurons from focal ischemic injury (64). 这样，葡萄糖载体基因的过度表达通过疱疹单一腺病毒载体进入到神经系统，保护神经元不受局部缺血损伤（64）。
Thus, overexpression of the glucose transporter gene via the introduction of a herpes simplex viral vector into the nervous system protected neurons from focal ischemic injury(64). 在其他的研究中，研究人员用携带腺病毒载体把"治疗"基因带入中枢神经系统。
One study has already shown that it is possible to inject a viral vector for the vasopressin receptor into the brains of the fickle meadow voles and make them better partners and parents. 一项研究显示：将一种抗利尿激素受体的病毒载体注射入“花心的”草甸田鼠的大脑中，会使它们成为更好的伴侣和父母。
The Asklepios researchers plan to insert a proprietary miniaturized version of the dystrophin gene into an adeno-associated viral vector designed to deliver the gene to human muscles. 目前没有使肌肉停止病变和逆转的有效的治疗方法。
Vector tropism is a research hot spot in cancer gene therapy, and targeted viral vectors play a key role in the enhancement of safety and efficiency in cancer gene therapy. 病毒载体的靶向性问题是肿瘤基因治疗中的重要研究热点,靶向病毒载体是提高肿瘤基因治疗安全性和有效性的重要途径。
In other studies, viral vectors have been used to introduce "therapeutic" genes into the central nervous system. 在其他的研究中,研究人员用携带腺病毒载体把"治疗"基因带入中枢神经系统。
The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR. 病毒RNA分子拷贝数和靶基因mRNA水平用实时定量RT-PCR确定。
It is often unpleasant to see an anomaly animal. 见到一只畸形动物常常会使人感到不快。
Pseudotyped viral vectors will provide novel opportunities for the study of gene therapy. 假型病毒载体将会给基因治疗研究带来新的机遇。
The problem: The gene for dystrophin is about 600 times larger than the viral vectors used to ferry the healthy genes into diseased cells. 困难在于，生产dystrophin的基因比用来传运健康基因到患病细胞内的病毒载体大大约600倍。
The animal is not used to the hotness of its new habitat yet. 这个动物还不习惯新栖息地的炎热。
Nonviral gene vectors have many inherent advantages over viral vectors in terms of safety ,immunogenicity and ease of manufacture. 非病毒的基因载体比病毒性基因载体具有高安全性、低免疫原性及易于生产的特点。