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Construction for retroviral expression vector of high expression and easy isolation of human hBD2 人hBD2高表达易分离逆转录表达载体构建
Kitts P A,Possee R D.A method for producing recombinant baculovirus expression vector at high frequency(J). 张志芳张颖吕鸿声等.;家蚕核多角体病毒解旋酶基因的克隆及部分序列分析（J）[J]
Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题：图1.;穿梭表达载体pRL_hEGF的构建。
Cell cycle of SiHa cells may be arrested by high expression of DCN. DCN高表达对SiHa细胞增殖周期有明显阻滞作用。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector. 另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
A SV_(40) late replacement expression vector containing secreted neuraminidase cDNA was transfected into COS cells. High nenraminidase activity could be detected in the cell culture medium. 将含分泌型甲型流感病毒神经氨酸酶cDNA的SV_(40)晚期区段取代载体转染COS细胞后,可在细胞培养液中测出高酶活力。
Giving is the highest expression of potency. 给予是力量的最高表现。
And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
High expression of plasma Fg is, on the one hand, easy to form thrombus and on the other hand, it promotes AS. 血浆Fg 的高表达一方面容易形成血栓,另一方面可促进AS。
The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
MTBE could induce high expression of c myc gene,and had no effect on the expression of GST P gene. 点杂交结果显示，MTBE可明显诱导大鼠肝组织中c－myc基因的高表达，而对GST－P基因的表达未见明显影响。
Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
The high expression of DDH and VEGF may play an important role in the pathogenesis of NSCLC. DDH与VEGF高表达可能与NSCLC发生发展有关。
Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
DPSCs may participate in the processes of wound repairing via high expression of MMP3. 高表达MMP3可能是真皮多能干细胞参与创伤修复的途径之一。
Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
The results indicated that the pIL-6 had high expression efficiency and was able to detected with ELISA. ELISA结果证实了所表达出的蛋白为猪IL-6，复性后具有一定活性，并测出了复性后活性蛋白的浓度。本研究成功获得猪IL-6基因并表达出该蛋白，为新型免疫增强剂的研制、开发与应用奠定了基础。