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- Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus. 本实验首先确定潮霉素浓度对香菇和平菇菌丝和原生质体再生的影响。
- T he concentration and method of hygromycin selection are determined through contr ast experiment. 通过比较试验，确定了潮霉素筛选的浓度和方法。
- The hygromycin is suitable to selection of castor transfomants while castor embryo axis is insensitive to kanamycin. 蓖麻胚轴对卡那霉素不敏感，潮霉素是蓖麻转化的适宜筛选剂。
- The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. ）幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
- Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. 潮霉素B是一种氨基糖甙抗生素，它通过干扰转位和促进80S核糖体上的错译从而抑制蛋白质的合成。
- Results showed that Micromonospora sp. 40027 was sensitive to thiostrepton,apramycim,streptomycin and capreomycin,and resistant to kanamycin, hygromycin and gentamycin. 而对卡那霉素、潮霉素和庆大霉素表现抗性。
- Utilizing this system, more than 50% hygromycin resistance selection efficiency was obtained with callus derived from young embryo (DREW, japonica ) and young panicle (D Shan B, indica ) as receptor, some regenerated plants were acquired. 依据该体系,以粳籼DREW 的幼愈伤组织和籼稻D汕B的幼穗愈伤组织为受体,经农杆菌AGL1/pCAMBIA1301转化后, 获得了超过50%25 的潮霉素抗性愈伤筛选率, 并获得再生植株。
- This effect makes it possible to eliminate all tumor cells despite only a fraction of them expressing HSV1 -tk gene. In this study, A fusion gene encoding both HSV1-TK and hygromycin phosphotransferase was used as the therapeutic gene. 本研究用一种与潮霉素磷酸转移酶基因(hygromycinphosphotransferase gene，hph)相融合的HSV1-tk基因作为目的基因(称为HyTK基因)，构建了重组逆转录病毒载体LNCHyTK；
- By the means of leave assay of hygromycin resistance, PCR amplification of a HPT fragment and Southern blot, it showed that RCOI and OsAOS fragments were successfully inserted into the rice genome. 利用离体叶片潮霉素快速鉴定、潮霉素PCR和Southern杂交等方法对转基因植株后代进行鉴定，证明了RCOI和OsAOS基因的片段正确插入水稻基因组；
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 标题： 图1 Rb反义基因表达载体结构示意图此表达载体Rb的转录方向与35S启动子的转录方向相反,并且含有GUS报道基因和抗潮霉素基因。
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系，对一些重要的影响银杏转化的因素，包括外植体类型，选择标记和筛选压，农杆菌工程菌株，共培养时间和恢复时间第一次进行了试验并优化了条件。
- The time for hygromycin selecting 筛选时间
- hygromycin B phosphotransferase gene 潮霉素磷酸转移酶
- Hygromycin B phosphofransferose gene 潮霉素B磷酸转移酶基因
- Study on selection efficiency of rice calli with hygromycin and PPT 潮霉素和PPT对水稻愈伤组织筛选效果的比较
- Stability of hygromycin resistant transformant of Colletotrichum lagenarium A, 潮霉素抗性转化子的稳定性
- Keywords Aspergillus fumigatus;Agrobacterium tumefaciens;Hygromycin resistance; 烟曲霉;根癌农杆菌;潮霉素抗性;
- Keywords Hygromycin B phosphotransferase;Monoclonal antibody;Identification; 单克隆抗体;鉴定;
- Keywords Hygromycin;Resistance;Feeder;BDF1 mice;transgenic;Model;animal; 潮霉素;饲养层;小鼠;BDF1;转基因;模型;动物;
- homomycin 匀霉素
