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- We can get nonmalignant hybrid cells. 我们可以获得非恶性的杂种细胞。
- Pure red cell aplasia with thymoma: evidence of T cell clone disorder. 胸腺瘤致单纯红细胞再生障碍机制的初步探讨。
- A hybrid tulip; a hybrid cell. 杂交郁金香; 杂交细胞
- Interspecies cloning is a new technology with the development of Somatic cell clone technology. 异种克隆技术是在体细胞克隆技术成功后发展的一项新技术。
- After the plasmid EGFP-C1 was transfected into INPC, the stable cell clone designated INPC/EGFP was isolated by G418 selection. The positive rate of EGFP was observed. 质粒EGFP-C1转染INPC后;经G418筛选;挑选细胞克隆;命名为INPC/EGFP;观察其EGFP阳性率.
- The hybrid cells lines were demonstrted as hybridoma by karyotyping. 经染色体核型分析证实为杂交瘤细胞。
- This review introduce the present situation in the technique progress, the basic theoretic research and the applications of the technology of somatic cell cloning. 本文对哺乳动物体细胞克隆技术研究现状、理论基础研究、应用等方面作一综述。
- A hybrid tulip;a hybrid cell. 杂交郁金香;杂交细胞
- Methods T cell clones were isolated by limiting dilution. 方法通过有限稀释法建立T细胞克隆。
- It may survive and give rise to two mononucleate hybrid cells called synkaryons. 可存活并产生两个称为合核体的单核的杂种细胞。
- Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen. 目的探讨在建立了分泌抗鼠疫F1抗原的单克隆抗体杂交瘤株后,对其进行克隆化的实验方法。
- This may require isolating a very large number of hybrid cells in order to obtain but a few clones. 这也许需要分离出一大批杂种细胞以获得只是小量的纯系。
- Methods:Using cell clone tech nique,cell elect rophoresis and flow cytometer,we establish ed three sublines with different metast atic potential from human ovarian cancer li ne-SKOV3 (S1,S6 and S14). 方法:应用有限稀释法对卵巢癌细胞系SKOV3进行单细胞克隆,共分离出14个克隆亚系,根据细胞电泳率,流式细胞仪检测所得细胞周期,筛选出3个电泳率差异较大的克隆亚系(S1、S6和S14)。
- The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer. 本研究是第一次报道在体细胞克隆和胚胎移植后成功应用嘌呤霉素标记获得了转强化型绿色荧光蛋白(EGFP)基因的小猪。
- Shanghai申友biotechnology company completed the study of "primer synthesis and gene sequencing," the work of the OPG (-/+) successful heterozygous mouse embryonic stem cell cloning. 上海申友生物技术公司完成了该研究中“引物合成和基因测序”的工作,成功获得了OPG(-/+)杂合子小鼠胚胎干细胞克隆。
- RT-PCR showed that hepG2 cells were positive for B7-1 at the messenger RNA(mRNA)level. The hepG2/Hb7-1 cell clone strongly expressing B7-1(positive rate:92.2%)was picked out. 而HepG2 /hB7 1的B7 1分子阳性表达率达到 92 6%25 ,转染B7 1基因后较转染前增大了 2 8倍多。
- Recent progress in handmade somatic cell cloning 徒手切割法体细胞克隆研究进展
- The hybrid offspring of a zebra and a horse. 杂交斑马斑马与马杂交的后代
- Hybridized cell lines ( CNf-A/B95-8 ) were obtained by hybridization of CNf-A cell with B95-8 cell and EBNA was detected in these hybridized cell lines. HAT培养基能杀死CNf-A细胞。 此细胞能与带EB病毒基因的类淋巴母细胞B95-8融合,形成的杂交细胞能在HAT培养液中存活,并可检出EB病毒的核抗原。
- Only a fraction of 15% of the T cell clones presented obvious HBcAg-specific proliferation response. 仅15%25 (6/40)的T细胞克隆呈现明显的HBcAg特异性淋巴细胞增殖反应。