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Keywords condyloma acuminata;human papillomavirus;peripheral blood mononuclear cell;late gene;major coat protein;baculovirus expression system;enzyme-linked immunosorbent assay; 尖锐湿疣;人乳头瘤病毒;外周血单一核细胞;晚期基因;主要衣壳蛋白;昆虫-杆状病毒表达系统;酶联免疫吸附法;
CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 云南省烟草花叶病毒和黄瓜花叶病毒外壳蛋白基因克隆及序列分析。
The phylogenic tree of PLRV isolates was established according to the amino acid sequence of coat protein. 依据PLRV外壳蛋白氨基酸序列建立了PLRV不同分离物的系统进化树。
The ScFv gene was expressed as fusion protein with phage g3p coat protein and displayed on the phage surface. 结果通过免疫荧光鉴定，这种抗体仍保留着亲代抗体的特性，能与登革3型病毒发生特异性结合。
This gene codes for the coat protein of a bacteria-infecting virus, or bacteriophage. 这是一种感染细菌的病毒（噬菌体）表面蛋白的基因。
The inhibition of plant activator protein on RNA and coat protein of tobacco mosaic virus(TMV) was studied. 研究了植物激活蛋白对烟草花叶病毒RNA及外壳蛋白的抑制作用。
Detection of IgM Antibodies to Coxsackie B Viruses in Viral Myocarditis by Using a Synthetic Pep- tide Antigen of CVB Coat Protein VP1. 应用VP1蛋白的合成肽抗原检测心肌炎患者血清中柯萨奇B病毒IgM抗体
Although the virus coat protein was present in the endosperms of the seeds of infected wisteria plants by ELASA tests, it is not seed-borne. 虽然在发病紫藤种子的胚乳中能检测到其外壳蛋白的存在，但此分离物却不能通过种子传播。
It appeared isometric with a diameter of 25 nm,had its coat protein with MW 24 700 dalton,contained three double strand RNA species that is 3.8 kb,1.4 kb,1.1 kb respectively. 病毒呈二十面体 ,直径约 2 5nm ,有空心和实心 2种粒子 ,具有分子量为 2 4 7kd的外壳蛋白和长度分别为 3 8kb、1 4kb、1 1kb的 3个双链RNA组分。
However, other scientists are skeptical and believe that the prion protein is a viral coat protein that protects a small nucleic acid that has not yet been detected. 然而,另外的科学家表示怀疑，他们相信普利昂蛋白是病毒中用于保护至今还未被检测到小核酸的衣壳蛋白。
But it need long period and is apt to be limited by season.Based on coat protein (CP) of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. 血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备杭血清需时间长，对含量少和无蛋白外壳的病毒无法检测；
A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. 以分离自马铃薯主栽品种“紫花白”的马铃薯卷叶病毒(PLRV)分离物的RNA为模板,用人工合成的引物,用反转录和随后PCR扩增的方法合成了PLRV外壳蛋白(CP)基因的cDNA,并克隆于pUC19中。
Transgenic tobacco (Nicotiana tabacum L. cv.Xanthi) plants which express coat protein (CP)gene (cp) of potato virus X (PVX) were generated via Agrobacterium tumefaciens-mediated gene transfer. 利用PCR方法获得了马铃薯X病毒(PVX)外壳蛋白(CP)基因(cp);并将其构建到植物表达载体中;利用农杆菌介导的叶盘法转化烟草(Nicotiana tabacum L.;)
The coat protein gene of GPV, a Chinese serotype isolate of BYDV, was used to construct three plant expression vectors, pPPI 2, pPPI 3 and pPPI 5, which contain Act promoter or Emu promoter respectively. 以我国特有的大麦黄矮病毒 GPV株系的外壳蛋白 ( CP)基因为材料 ,设计合成了分别含有 Act启动子或 Emu启动子的植物表达载体 p PPI2、p PPI3和 p PPI5。
The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively. 设计3对表达引物分别扩增侵染了百合无症病毒(lily symptomless virus,LSV),百合斑驳病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外壳蛋白基因,并在大肠杆菌BL21(DE3)plysS中原核表达。
The skirmish grew into a major battle. 小冲突扩大形成大战斗。
Peanut stripe virus-Hongan isolate was obtained from the infected peanut seedling in Hongan County,Hubei Province. Its coat protein gene was amplified by RT-PCR and cloned into pGEM-T Easy vector. 从湖北省红安县花生种传苗中得到花生条纹病毒(Peanut stripe virus,PStV)分离物(PStV-Hongan),提取感病叶片的总RNA,RT-PCR扩增了其外壳蛋白基因(cp),克隆至pGEM-T Easy载体,转化大肠杆菌并鉴定。
Some slight incident may touch off a major war. 某个小事件可能会引发大战争。
Surprisingly, some isolates accumulate to high levels but still can’t be detected by ELISA.Sequence analysis revealed distinctive amino acids changed in the coat protein ORF of these isolates. 本论文首先探讨酵素连结免疫抗体法准确度的限制因子，发现病毒含量过低或是鞘蛋白胺基酸序列有变异，皆为造成ELISA侦测准确度不高之原因。
Local fighting might spark off a major war. 局部战斗有可能引发一场大规模战争。