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Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS|PAGE and stained using Con A|HRP. 用亲和层析，SDS?PAGE 及印迹技术，从玉米精细胞中分离纯化质膜蛋白。
MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. 方法 AchE单克隆抗体是一种IgG1抗体 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA亲和层析柱进行提取纯化 ,获得纯化的单克隆抗体Fab段。
Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 结果3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%25左右,Fab抗体柱纯化的Fab抗体的纯度为95%25,但这两种亲和柱的目的蛋白收率都不高,分别为35%25、55%25。
The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis. 以这些亲生物性溶胶管柱来作样品清理的应用潜力，已由环境、食物及医疗上获得证明。
She angled her column of chitchat toward teenagers. 她的漫谈栏侧重于十几岁的青少年。
She has a strong affinity for Beethoven. 她酷爱贝多芬的乐曲。
Peter has a special affinity for booze. 彼得嗜酒。
He felt a strong affinity to the Russian girl. 他深受那个俄国姑娘吸引。
He used to write a column for this newspaper. 他过去曾为这家报纸写专栏文章。
She feels a strong affinity to him. 她感到他对她有很强的吸引力。
His column is syndicated throughout the world. 他的专栏文章通过报业辛迪加在世界许多报刊上发表。
Italian has a close affinity to French. 义大利语和法语很相似。
The column is surmounted by a statue. 柱顶上树立了一尊雕像。
Nelson's Column is a famous monument in London. 纳尔逊纪念碑是伦敦著名的纪念碑。
He always read the sports column in the Times. 他总是阅读《泰晤士报》的体育栏。
Keywords high performance liquid chromatography;immune affinity column;albuterol;plasma; 高效液相色谱法;免疫亲和柱;沙丁胺醇;血浆;
The top part of a pillar or column. 柱头一根柱子或圆柱的顶端部分
There is an affinity between Chinese and Japanese character. 汉字与日文之间有密切关系。
A column in the periodic table of the elements. 属，族元素周期表上的一栏
The column, four abreast, stretched out over one kilometre. 队伍4个人一行，长达1公里以上。