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- 以碘化丙锭(propidium iodide,PI)结合流式细胞术分析细胞周期分布情况,AnnexinV-APC/CFSE双染结合流式细胞术并使用DNA Ladder凋亡检测试剂盒分析细胞凋亡的情况。Distribution of cell cycle was analyzed by propidium iodide (PI) staining associated with flow cytometry. Cell apoptosis was analyzed by AnnexinV-APC/CFSE double stainging, flow cytometry and by method of DNA Ladder.
- 方法:采用Daniel方法建立神经元缺氧凋亡模型,加入补阳还五汤药物血清,应用碘化丙啶(propidium iodide,PI)染色法经流式细胞仪检测神经元凋亡率,免疫组化法流式细胞仪检测p53和p21的表达。Methods: The model of hypoxia neuron apoptosis was established adopting Daniel method and treated with BHD drug serum. The neuron apoptosis rate was determined by flow cytometry with propidium iodide staining, the p53 and p21 gene expression was tested by immunohistochemical method with flow cytometry.
- 方法将烧伤血清和痂下水肿液与人脐静脉内皮细胞一同孵育12、24 h后,采用Annexin-V-Fluos(A-V)和碘化丙啶 (Propidium iodide,PI)标记内皮细胞,流式细胞仪检测其凋亡和坏死百分率。Methods Human umbilic vein ECs cultured in vitro and stained with annexin-V-Fluos (A-V) and propidium iodide (PI) were incubated with peripheral blood serum or STF collected from patients with severe burn injuries, and apoptosis and necrosis of the EC were observed by means of flow cytometry.