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- Donors and recipients HLA class I typing was performed using complement dependent cytotoxicity (CDC) test with special monoclonal tray (SMT) and HLA class II gene typing by micro sequence specific primers polymerase chain reaction (Micro PCR SSP). 采用补体依赖性细胞毒试验 (CDC)和微量序列特异性引物聚合酶链反应 (Micro PCR SSP)技术进行HLA I类和II类分型。
- Sequence specific primer ( PCR - SSP) 顺序特异性引物
- The sequence specific primers of the gene mutation of the mannose combining agglutinator gene exon I at 52 site condon was detected with polymerase chain reaction. 进行甘露糖结合凝集素外显子I52位密码子基因突变的序列特异性引物聚合酶链式反应检测。
- Polymerase chain reaction- sequence specific primer 聚合酶链反应-序列特异引物法
- Sequence specific primer based polymerase chain reaction, PCR-SSP 运用序列特异性引物聚合酶链反应
- Polymerase chain reaction with sequence specific primer, PCR-SSP 应用聚合酶链反应-序列特异性引物方法
- A~(1,2)BO~(1,2) genotyping by PCR sequence specific primer PCR-SSP法检测A~(1,2)BO~(1,2)血型基因型
- polymerase chain reacation sequence specific primer (PCR-SSP) 聚合酶链反应-序列特异性引物（PCR-SSP）
- Methods HLA?DQA?1 and DQB?1 gene polymorphism was tested in 30 CLT patients and 24 normal controls of Chinese Han nationality by using DNA amplification with polymerase chain reaction of sequence?specific primers (PCR?SSP). 方法用序列特异性引物的聚合酶链反应（PCR?SSP）方法，分析30例由病理证实的我国汉族CLT患者及24名正常对照的外周血白细胞基因组DNA的HLA?DQA1及DQB1位点的基因多态性分布。
- Evaluating the polygene polymorphism by sequence specific primers polymerase chain reaction in large samples 用序列特异性引物多聚酶链反应的方法进行大样本、多基因的多态性鉴定
- Keywords HLA-DRB1;polymerase chain reaction;sequence specific primers;genotyping;Lahu Chinese; 关键词HLA-DRB1;聚合酶链反应;序列特;异引物基因分型;拉祜族;
- The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. 采用PCR-序列特异性引物技术(SSP)对HLA-DQB作中分辨法分型。
- Keywords sequence specific primer;polymerase chain reaction;genotyping polymorphism; 序列特异性引物;多聚酶链反应;基因多态性;
- sequence specific primers 序列特异性引物
- Two pairs of specific primers: Tch old and Tch new primers were designed based on the result of 18S rRNA gene sequence. 经试验证明这两对引物能够非常有效的区分吕氏泰勒虫和尤氏泰勒虫。
- sequence specific primer 序列特异的引物
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence. 根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
- The ORF1 sequence of gene mrp encoding muramidase-released protein(MRP) was amplified from genomic DNA of Streptococcus suis type 2 Qinghai strain by PCR with specific primers. 根据猪链球菌2型溶菌酶释放蛋白基因(mrp)的序列,设计并合成了1对特异性引物,以青海株的基因组DNA为模板扩增了mrp基因ORF1序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素（LT）的基因序列设计引物，用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
