您要查找的是不是：
- A and B fragments of CYP2J3 gene were connected by overlap extension PCR to obtain ORF sequence of CYP2J3 gene. 运用重叠延伸聚合酶链反应连接CYP2J3基因A和B两片段,扩增出大鼠CYP2J3基因全长ORF序列。
- In order to improve the optimum temperature of lipases, The Penidilum expansuin lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. 利用重叠延伸PCR法对扩展青霉碱性脂肪酶(PEL)基因作了体外定点突变，获得了最适作用温度有所提高的突变体。
- Methods The Ecs gene was cloned using overlap extension PCR and the condon of Met28 was mutated to Leu. Three pieces of Ecs genes were linked in the same orientation and inserted into expression vector pET30a. The recombinant plasmid was expressed in E. 方法:采用重叠延伸PCR克隆Ecs基因;将第28位Met的密码子突变为Leu;利用其序列特点及酶切位点;在表达载体pET30a上将Ecs基因以同向串联方式连接;在E.
- Designed and synthesised 4 fragments of antibacterial peptide B gene, then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing). 摘要人工设计并合成了抗菌肽B基因的4个寡聚核苷酸片段，通过重叠区扩增法，扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。
- Gene splicing by overlap extension, SOE 作了改进,在拼接延伸后再进行一次
- Overlap extension polymerase chain reaction 重叠延伸PCR
- overlap extension by polymerase chain reaction 重叠延伸PCR法
- splicing by overlap extension (SOE) 重叠延伸技术（SOE）
- splicing by overlap extension PCR 重叠延伸PCR（SOEPCR）
- splicing proliferation by overlap extension PCR重叠延伸技术
- Gene splicing by overlap extension (SOEing) 重叠区扩增基因拼接法（SOEing）
- gene splicing by overlap extension 重叠区扩增基因拼接法
- gene splicing by overlap extension PCR 重叠延伸PCR
- PCR splicing by overlap extension 重叠伸展的PCR缝接
- Over the twenty years, it has branched into three characteristic strategies: splicing by overlapping extension (SOE), jumping polymerase chain reaction (JPCR) and DNA shuffling. 经过二十多年的发展，该技术已形成三个各具特色的方法分支：重叠延伸拼接、跳跃PCR和DNA重排。
- The letter was written on a single sheet of paper. 这封信只用一张纸写成。
- Keywords Th cell epitop;DNA vaccine;splicing by overlap extension; 辅助细胞表位;DNA疫苗;互补延伸拼接;
- One single, second class, to winchester, please. 买一张去温撤斯特单程二等票。
- He got over the stream with a single leap. 他一跃就跳过了小溪。
- The bridge crosses the river in a single span. 河上那座桥是单跨桥。
