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- offers by tender (英国发行新证券的方式之一)标售发行法
- 本文讨论了三种 DDBMS 组成方式:松散结合型(loose cou-pling)、紧密结合型(tight coupling)和集成型(integrat),并对它们的优缺点进行了分析。There are three DDBMS architectures-loose coupling,tight coupling and integrat,which will be dis- cussed in the section 2.
- 方法:采用DNA重组技术构建真核表达质粒pTRE-Tight-CFP-CD11b和pTRE-Tight-YFP-CD18(CD11b和CD18是Mac-1的两个亚基),通过脂质体介导共转染CHO细胞,用荧光显微镜观察转染后阳性细胞;Methods: The eukaryotic expression vectors pTRE-Tight-CFP-CN11b and pTRE-Tight-YFP-CD18 were constructed by recombinant DNA technique. The 2 vectors were co-transfected into CHO cells using liposome to fuse CD11b and CD18: the 2 subunits of Mac-1. Fluorescence microscope was used to observe the cyan fluorescence and the yellow fluorescence of Mac-1-FP.
