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- DNA methylation plays a key role in transgene silencing. DNA甲基化是引起外源基因沉默的主要原因。
- The indirect approach seeks second-hand signs of the transgene or its vector. 间接方法寻求找到转基因或者其载体的二手信号。
- The mechanisms of gene silencing include copy numbers and configuration of transgene, integration sites in plant and transcription of transgene, etc. 基因沉默的机制是多方面的,包括转基因的拷贝数和构型、在植物上的整合位点、转基因的转录水平等。
- Isolation and cloning of human neural stem cells, intracerebral grafting and transgene expression in mice. 人神经干细胞的分离、克隆和动物脑内移植及转基因表达。
- AAV-mediated striatal transgene expression of TH, AADC and GCH is more effective for gene therapy on Parkinsonian rat. AAV载体介导的TH、AADC和GCH三重基因纹状体内共表达对于帕金森病大鼠的基因治疗更为有效。
- Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development. 转生长激素基因促进鲤鱼胸腺的发育。
- The invention discloses a method and the application to build ground peanut transgene acceptor system. 本发明公开了一种建立花生转基因受体体系的方法及应用。
- Spiker S, Thompson W F.Nuclear matrix attachment regions and transgene expression in plants. 颜敬昌.;植物组织培养手册
- The conjugation of histidine onto helper lipid enhances transgene expression contrast with unmodified liposomes. 而经由组氨酸修饰后的微脂粒相对于未修饰之微脂粒,其能增进基因表现效率。
- Transgene silencing is one of the most important reasons that transgene can not express. 摘要转基因沉默是导致外源基因不能在转化植株中正常表达的重要原因。
- Conclusion AAV-mediated striatal transgene expression of TH, AADC and GCH is more effective for gene therapy on Parkinsonian rat. 结论AAV载体介导的TH、AADC和GCH三重基因纹状体内共表达对于帕金森病大鼠的基因治疗更为有效。
- Bawden C S,Powell B C,Walker S K.Expression of a wool filament keratin transgene in fiber alters structure[J].Methods in Enzymol,1987,(155):397-415. 姚金波;何天虹;何美劲;等.;还原C法制备羊毛角蛋白质溶液的工艺优化[J]
- There was no significant difference in the response of glucose-stimulated insulin release between the transgene group and the control group (P>0.05). 且葡萄糖刺激胰岛素释放反应与对照组无明显差异(P>0.;05)。
- Studies on transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat. 优质HMW谷蛋白亚基转基因小麦的获得及其遗传稳定性和品质性状分析。
- Hu, Y.-C., Lo, W.-H. Development of a novel baculovirus vector that enables prolonged transgene expression. US patent application filed. 胡育诚;罗文鑫.;“具备长期表现能力之新式重组杆状病毒载体”;中华民国发明专利申请中
- At present cultivates the first batch breast milk ferric albuminate iron albuminate transgene cow entered the lactation period. 目前培育的首批人乳铁蛋白转基因奶牛已进入泌乳期。
- To study the mechanism of transgene's a integration in the host genome, first stop is to clone the integration site sequence. 摘要转基因动物整合位点克隆及相关序列特徵研究是探索外源基因整合机制和整合稳定性的重要手段。
- Site-specific recombination technology provides an important means for regulating transgene expression and improving efficiency of gene transformation. 摘要定点重组转基因技术是调节外源基因表达,提高转基因效率的重要手段之一。
- The expression of GFP for lines D and E was abnormal and may be related to the physical location of the transgene or the gene construct used. D和E株系的GFP表达不正常可能和gfp基因插入位置或基因的结构有关.
- Transduced cells were cloned in G418 and expanded for analysis of IGF-1 transgene expression and its effect on the cell proliferation. 方法 以脂质体为载体,用携带有报告基因绿色荧光蛋白(Green fluorescent protein,GFP)的重组IGF-1转染体外培养的人RPE细胞系,经G418筛选,通过荧光显微镜观察转染情况;