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- 农杆菌LBA4404Agrobacterium LBA4404
- 根癌农杆菌LBA4404agrobacterium tumefaciens strain LBA4404
- 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB),与含绿色荧光蛋白基因(gfp)和潮霉素磷酸转移酶基因(hot)的pcAMBIA 1300-SmGF、P的根瘤农杆菌LBA4404共培养,培育出了转基因的蝴蝶兰。Transgenic orchid (Phalaenopsis cv. White Hikaru) plants were regenerated by inoculating a needle wounded protocorm-like body ( PLB) with Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA 1300-SmGFP that carries green fluorescent protein gene (gfp) and hygromycin phosphotransferase gene ( hpt) .
- 把该反义片段连接至pBI121双元载体质粒上得到CaMV35S组成型启动子的表达载体pBI35S-AMF,随后利用"三亲杂交"法将pBI35S-AMF质粒转入农杆菌LBA4404和EHA105两个菌株中.The fragment was introduced to pBI121 generating pBI35S-AMF which contained CaMV35S promoter, and then pBI35S-AMF was transferred into Agrobacterium tumefaciens strain LBA4404 and EHA105 through the assistant plasmid pRK2013/HB101 by triparental cross.
- 见难而上
- 农杆菌Agrobacterium tumefaciens
- 根癌土壤农杆菌Ti质粒毒性区VirD1基因的克隆及序列分析Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens'Ti Plasmid
- 发根农杆菌Ri质粒及其在植物次生代谢产物研究中的应用The Ri Plasmid of Agrobacterium rhizogenes and Its Application to Plant Secondary Metabolites
- 农杆菌法Agrobacterium-mediated transformation
- 离体培养植物细胞的表面与农杆菌介导基因转化频率的研究Relationship Between the Outer Surfaces of Plant Cells Cultured in vitro and the Frequencies of Transformation Agrobacterium-mediated
- 农杆菌渗入agroinfiltration
- 发根农杆菌Agrobacterium rhizogenes
- 农杆菌介导Agrobacterium-mediated
- 根瘤农杆菌Agrobacterium tumefaciens
- 农杆菌转化Agrobacterium-mediated transformation
- 农杆菌注射injection of Agrobacterium
- 残存农杆菌Agrobacterium
- 放射农杆菌Agrobacterium ractidoacter
- 野生型农杆菌Wild Type Agrobacteria
