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- 方法用PCR-southern blot方法检测30株人型结核分枝杆菌及4株非人型结核分枝杆菌(牛型分枝杆菌、勘萨斯分枝杆菌、胞内分枝杆菌、鸟分枝杆菌)的TB-DNR,分别与人型结核分枝杆菌H37Rv标准株作对照。Methods Detecting the TB-DNA in 30 strains of Mycobacterium tuberculosis and 4 strains of non- Mycobacterium tuberculosis by PCR-southem blot, and comparing with that of the reference strain (H37Rv).
- 本论文的目的是:(1) 利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因;The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction (PCR) from the genomic DNA ofM. tuberculosis H37RV strain;
- 结核分枝杆菌H37RvMycobacterium tuberculosis H37 Rv
- 构建了高效表达结核杆菌H37Rv ICL的质粒;We constructed the plasmid which could highly express Mycobacterium tuberculosis H37Rv ICL.
- 二聚体蝎型探针荧光定量PCR快速检测临床标本结核分枝杆菌DNA Rapid detection of Mycobacterium tuberculosis DNA by fluorescent quantitative PCR assay using duplex scorpion primers
- 单链构象多态性与核酸杂交联合检测耐氧氟沙星的结核分枝杆菌Polymerase chain reaction-single strand conformation polymorphism analysis combined with nucleic acid hybridization to detect ofloxacin-resistant Mycobacterium tuberculosis mutation selected in vitro
- 结核分枝杆菌Ag85B-Ag85A双抗原融合真核表达质粒的构建及表达Construction of the fused eukaryotic expression vector of Mycobacterium tuberculosis Ag85B and Ag85A antigens and its expression
- 方法采用PCR方法从结核分枝杆菌H37Rv基因组DNA中扩增出RpfE基因,双内切酶消化后,与同样酶消化的pET32a(+)载体连接,转化大肠杆菌Top10。Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E.
- 结核分枝杆菌L型检测的临床意义Clinical significance of the examination to MTB-L
- 结核分枝杆菌培养和药敏试验及菌群鉴定快速荧光法的研究Study on a rapid fluorometric method for culture,drug susceptibility testing and identification of group in Mycobacterium tuberculosis
- 分枝杆菌结核Mycobacterium tuberculosis
- 分枝杆菌,非结核Nontuberculous mycobacteria
- 扩增Rv2032和Rv3127基因从结核分枝杆菌H37Rv菌株基因组数据库 (http://genolist. pasteur.fr/TubercuList/)中获得Rv2032和Rv3127 基因的核苷酸序列。Amplified Rv2032 and Rv3127 genes from M. tuberculosis genomic DNA. DNA sequences of Rv2032 and Rv3127 genes were acquired from M. tuberculosis genome database (http://genolist.pasteur.fr/TubercuList/).
- 结核分枝杆菌ICL mRNA特异性10-23脱氧核酶切割活性的鉴定及其切割特点Cleavage Activity and Characteristics of 10-23 Deoxyribozyme Targeting ICL mRNA of Mycobacterium tuberculosis
- 猫爪草提取物对结核分枝杆菌临床分离株的可能作用靶标Identification of Differential Expression Proteins of Mycobacterium tuberculosis Strain Isolated from Clinical Species Treated with Radix Ranuncoli Ternati Extracts by Comparative Proteomics
- 非结核分枝杆菌nontuberculosis mycobacteria
- 结核分枝杆菌L型Mycobacterium Tuberculosis L-form
- 结核分枝杆菌DNAMycobacterium tuberculosis DNA
- 多重聚合酶链反应-单链构象多态性分析检测耐异烟肼结核分枝杆菌Study on isoniazid-resistant Mycobacterium tuberculosis isolates by multiple-polymerase chain reaction-single strand conformation polymorphism
- 非结核分枝杆菌病Non-tuberculous mycobacterial diseases
