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Agrobacterium tumefaciens (Smith and Townsend) Conn. 冠瘿病
High Transformation System of Cotton Mediated by Agrobacterium tumefaciens. 棉花农杆菌介导高效转化体系。
The growth of Agrobacterium tumefaciens could be inhibinated effectively by 500 mg/L Carbenicillin. 500.;0mg/L的羧苄青霉素（Carb）浓度能够有效地抑制根癌农杆菌在培养基中的增殖；
In this paper, we studied the production and the metabolic regulation from Agrobacterium tumefaciens KY-3085. 本文对根瘤土壤杆菌（Agrobacterium tumefaciens KY-3085）发酵制备辅酶Q10的发酵条件及代谢途径进行了研究。
Antisense CCoAOMT cDNA and 4CL cDNA had been transformed into triploid Chinese white poplar mediated by Agrobacterium tumefaciens. 通过农杆菌(Agrobacterium tumefaciens)介导，将反义CCoAOMT cDNA和4CL cDNA转入三倍体毛白杨。
The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表达载体P13w4一cTB和pcWIJN分别转化感受态根瘤土壤杆菌，经过PcR筛选和酶切鉴定，获得根瘤土壤杆菌转化子。
Agrobacterium A genus of soil bacteria, the species A. tumefaciens being the causative agent of CROWN GALL, a type of tumor in plants. 根癌农杆菌：土壤中存在的一种农杆菌，可以感染双子叶植物的受伤组织诱发一种植物的肿瘤-冠瘿瘤。
Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. 将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ;得到植物表达载体p35EZ。通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .;)
Preculturing of immature cotyledon was beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. 体细胞胚的脱水处理显著促进“中黄4号”体细胞胚的萌发。未成熟子叶的预培养有利于根癌土壤杆菌感染子叶外植体体细胞胚的分化。
After elucidation the mechanisms of Agrobacterium tumefaciens itself when they infected plant cells, researchers have turned their interest to factors of plant at this process. 随着农杆菌介导遗传转化过程中农杆菌一方转化机理的阐明，人们现在已经将目光转向了参与在农杆菌介导遗传转化过程中的植物因子。
The paper summarized the application of agrobacterium tumefaciens mediated,particle bombardment,ovary injection and pollen tube pathway method in maize transformation. 本文就近几年来根农癌杆菌介导法、基因枪法、子房注射法、花粉管通道在玉米基因转化中的应用情况加以综述.
Based on this highly efficient shoot induction, the application of filter paper to the genetic transformation of Artemisia annua L. by Agrobacterium tumefaciens was investigated. 在高效丛生芽诱导体系的基础上，探讨了滤纸在根癌农杆菌介导的青蒿遗传转化中的应用。
Transformation of tobacco by SgNCED1 and its functional analysis Expression vector of pBI-SgNCED1 was constructed and introduced to tobacco by Agrobacterium tumefaciens. 3、SgNCED1基因转化烟草及功能分析构建了SgNCED1基因的表达载体pBI-SgNCED1，并用农杆菌介导的方法转化烟草（Nicotiana tabacum）。
The conditions of establishing the high regeneration system of Zoysiagrass were studied, the genes of IPT and CBF1 were transformed into the calli using microprojectile bombardment and Agrobacterium tumefaciens transformation. 我们在建立了高效的结缕草组培再生体系的基础之上,通过基因枪法和农杆菌介导法转化CBF_1和IPT基因,获得转化CBF_1基因的植株,为培育新品种打下基础。
Mediated by Agrobacterium tumefaciens GV3101(pMP90), Leaf discs of Nicotiana bethamiana were transformed by these 3 constucts. Tested by PCR, transgenic plants were obtained for each construct. 借助于根癌农杆菌(Agrobacterium tumefaciens)菌株GV3101(pMP90)转化白氏烟(Nicotiana benthamiana),经过PCR检测,获得转基因植株。
The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method. 克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。
The fragment was introduced to pBI121 generating pBI35S-AMF which contained CaMV35S promoter, and then pBI35S-AMF was transferred into Agrobacterium tumefaciens strain LBA4404 and EHA105 through the assistant plasmid pRK2013/HB101 by triparental cross. 把该反义片段连接至pBI121双元载体质粒上得到CaMV35S组成型启动子的表达载体pBI35S-AMF;随后利用"三亲杂交"法将pBI35S-AMF质粒转入农杆菌LBA4404和EHA105两个菌株中.
Betaine aldehyde dehydrogenase (BADH) cDNA cloned from Atriplex hortensis L. was transferred into tetraploid clone of black locust (Robinia pseudoacacia L.) by agrobacterium (Agrobacterium tumefaciens Conn) mediated transformation. 利用农杆菌(Agrobacterium tumefaciens Conn)介导法;将山菠菜甜菜碱醛脱氢酶(BADH)基因转入四倍体刺槐(Robinia pseudoacacia L.;)
The metabolites from three monocotyledonous and three dicotyledonous plants were respectively utilized to regulate the expression of virulence (Vir) genes in Agrobacterium tumefaciens. 本文研究了六种植物(三种单子叶植物、三种双子叶植物)愈伤组织的渗出物和抽提物对农杆菌Vir基因表达的调节作用。