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- What secondary structure predominates in this Fab fragment? 片段中的主要二级结构是什么?
- Expressed Fab fragment has the antigenic specificity for human erythrocytes. 于大肠杆菌表达了具有抗原结合特性的人抗-D抗体Fab段抗体分子。
- How many amino acid residues are in the heavy and light chains of the Fab fragment and in lysozyme? Fab片段的重链和轻链由多少个氨基酸残基组成,溶菌酶由多少个氨基酸残基组成?
- OBJECTIVE:To clone murine anti cTnI Fab fragment and analyse the nucleotide and deduced amino acid sequences. 目的:克隆鼠抗人cTnI单克隆抗体Fab段基因并进行序列分析。
- Ten strains that expressed soluble antibody Fab fragment were selected randomly. 随机挑取10株单克隆分别进行表达,将含有可溶性抗体Fab片段的细菌上清进行ELISA鉴定。
- Made sheep anti?human IgG Fab Sepharose 4B affinity chromatography, purified the souble Fab fragment. 制备羊抗人IgGFab抗血清、建立抗人IgGFab抗体-Sepharose4B亲和层析柱,纯化Fab片段。
- In this investigation, the anti-tumor efficacy of chimeric anti-CD20 Fab fragment antibody was analyzed by MTT assay. 为了研究嵌合抗CD20基因工程抗体Fab的抗肿瘤活性及其抗肿瘤机制,本研究采用竞争性免疫荧光抑制实验,证实抗CD20 Fab片段能竞争性抑制亲本鼠源性抗CD20单克隆抗体HI47和Daudi细胞CD20的结合。
- P3G9-L can express soluable Fab fragment and the function of Lac promotor is weak. 1.;P3G9-L为单Lac启动子同时驱动Fd段及K链的双顺反子的可溶性Fab段表达载体。
- Generation of human recombinant antibody Fab fragment to influenza virus from combined antibody library. 流感病毒中和性基因工程Fab抗体的研制
- Soluble human anti-keratin Fab fragment was successfully expressed in E. coli and efficiently purified by IMAC. 将利用噬菌体抗体库技术从半合成噬菌体抗体库中克隆到的人源性抗角蛋白抗体Fab片段成功地进行了可溶性表达。
- A complete murine anti cTnI Fab fragment was obtained in this study,which may provide basis for the production of the chimeric anti cTnI antibody. 本实验室获得了完整的鼠源性抗人cTnI单克隆抗体Fab段基因,为鼠抗人cTnI单克隆抗体的人源化改造奠定了基础。
- Objective:To obtain human mouse chimeric Fab fragment of the anti human small cell lung cancer mAb 2F7 by means of engineering antibody. 目的 :通过基因工程抗体改造获得具有与人小细胞肺癌有特异性反应的人 鼠嵌合抗体 2F7Fab片段。
- The constructor was cloned into phagemid vector pHEN-1-Fab/Hu for expressing the soluble form of chimeric SZ-58 Fab fragment in non-suppressor E. 表达的嵌合Fab片段为可溶性。
- AIM: To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus. 目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。
- In present study, the biologic function of anti-CD28 monoclonal antibody and its Fab fragment were investigated in vitro and in vivo. 在课题的第一部分,我们通过双向混合淋巴细胞培养实验发现,mAbCD28及其Fab片段可以有效地抑制混合淋巴细胞反应,其作用与剂量成正比。
- ForIon exchange Size exclusion column, purity and yield ratio of Fab fragment were very good, being 93.8% and 80% or more, respectively. 而离子交换柱纯化的Fab抗体的纯度为93.;8%25;经分子筛柱进一步纯化后;可达98%25以上;Fab抗体蛋白收率可达80%25以上。
- By ELISA, McAb 3A5 and the Fab fragment were strongly reactive with hepatoma BEL-7402 cells and weakly reactive with KB cells. 在瘤、心、肝、脾、肺、肾、胃、肌、肠中,每克组织注射剂量百分比单抗3A5高于Fab片段,但肿瘤组织与非肿瘤组织的比值(T/NT)则Fab片段高于单抗3A5。 说明虽然完整单抗3A5在瘤组织的绝对浓度高于Fab片段,但是Fab片段分布特异性高于完整单抗3A5。
- AIM: To clone and express a human monoclonal anti-D Fab fragment in E. coli and make benefits for the expression of the whole immunoglobulin molecules of anti-D. 目的:于大肠杆菌表达抗-D抗体Fab段基因,为真核载体表达完整的人抗-D抗体奠定基础。
- Methods By genetic engineering technology,human anti-keratin ScFv was constructed from Fab fragment selected from established semi-synthetic phage antibody library. 方法利用基因重组技术对从半合成噬菌体抗体库中克隆的人源性抗角蛋白抗体Fab片段进行改造,获得了人源性抗角蛋白ScFv片段,并进行DNA序列分析。
- Objective To develop monoclonal antibody Fab fragments using display technique. 目的 利用噬菌体抗体库技术制备抗甲状旁腺激素相关蛋白 (PTHrP)Fab抗体。