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- Objective:To establish fluorescence quantitative nested RT-PCR method for detecting Borna disease virus (BDV). 目的 :建立博尔纳病病毒 (BDV)荧光定量套式RT -PCR检测方法 ,为快速、准确地定量检测BDV奠定基础。
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- Detection deleted Duchenne muscular dystrophy carriers by fluorescence quantitative polymerase chain reaction. 应用荧光定量PCR检测缺失型DMD携带者的研究
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Objective:To construct recombinant plasmid pMD18-nm23-H1/p as the standards for nm23-H1 gene detection by real-time fluorescence quantitative PCR. 目的:制备用于nm23-H1基因mRNA实时荧光定量PCR检测的质粒标准品。
- Observation on the therapeutic effect of azithromycin on infant toxoplasmosis patients by fluorescence quantitative poly-merase chain reaction. 关键词:弓形虫病;阿奇霉素;聚合酶链反应;幼儿
- We measured hTERT mRNA expression in exfoliated cells in urine taken from 26 patients with TCC and 18 nonmalignant controls using real-time fluorescence quantitative RT-PCR. 应用实时荧光定量RT-PCR，定量检测26例膀胱移行细胞癌患者、12例良性泌尿系统疾病患者和6例正常人尿液中脱落细胞端粒酶亚单位hTERT mRNA的表达。
- METHODS Detecting MP,CP,Uu and CT in sputa of neonatal infants and MP and CP in throat swabs of children by the fluorescence quantitative polymerase chain reaction(FQ-PCR). 方法用荧光定量PCR法检测新生儿痰中MP、CP、Uu、CT,儿童咽拭子中MP、CP。
- Methods Pre-S1 antigen of hepatitis Bvirus with ELISA method, it is ultraviolet that ALT use LD method, HBV-DNA was determined by fluorescence quantitative PCR(FQ-PCR) assay. 方法乙肝病毒前S1抗原用ELISA方法检测,丙氨酸氨基转移酶用紫外-乳酸脱氢酸法检测,HBV-DNA测定运用荧光实时定量检测方法。
- Method:Serum samples of 106 suspected HBV patients were detected by MEIA and positive samples were then detected for HBV DNA by Fluorescence quantitative PCR. 目的:应用微粒子荧光酶免疫分析法(MEIA)定量检测乙肝病毒e抗原的浓度,以反映乙肝病毒感染的程度。
- Objective This study aimed to establish a fast and accuratequantitative method to detect Borna disease virus (BDV) ribonucleic acid(RNA) using fluorescence quantitative nested reverse transcriptasepolymerase chain reaction (FQ-nRT-PCR). 目的：本研究拟建立博尔纳病病毒(BDV)荧光定量套式逆转录酶聚合酶链反应(FQ-nRT-PCR)检测方法，为快速、准确地定量检测 BDV奠定基础。
- Methods Enzyme linked immunosorbent assay (ELISA) and fluorescence quantitative polymerase chain reaction (FQ PCR) technique were used to detect TOX specific antibodies and TOX DNA in the blood of pregnant women to diagnose maternal infection. 方法 用酶联免疫吸附法及荧光定量多聚酶链反应技术检测孕妇血中 TOX特异性抗体及 TOX DNA,对 TOX感染孕妇检测羊水或脐血诊断胎儿感染。
- Methods The P24 fragment of BDV RNA in peripheral blood mononuclear cells(PBMCs)was examined by fluorescence quantitative nested reverse transcriptase polymerase chain reaction(FQ-nRT-PCR)in 15 patients with multiple myeloma and 20 volunteers. 方法采用荧光定量套式逆转录聚合酶链式反应(FQ-nRT-PCR)检测了15例MM患者和20例健康对照组外周血单个核细胞(PBMCs)中BDV P24基因片段。
- HCV-RNA was detected by Fluorescence Quantitative PCR. Results 68.5% of IVDU had the HCV infection. One-way ANOVA showed that HCV infection was associated with the history of drug addiction, the use of contaminated needles and HIV/HBV infection. 结果静脉药瘾者中抗HCV阳性261人(68.;5%25);感染率与当地正常人群比较;差异有统计学意义(P<0
- Methods A model of animal experiment,a kit for sexdeter mining region Y (SRY) and a set of standard sample were developed for drawing standard curve by real time fluorescence quantitative PCR. 方法结合动物实验模型,设计性别决定因子Y(SRY)实时荧光定量聚合酶链反应(PCR)检测血细胞嵌合率的方法,建立质控标准品,使用实时荧光定量PCR技术,获得标准曲线。
- Methods: Fluorescence quantitative PCR (FQ - PCR) was used to detect quantitatively UU DNA and CT DNA in randomized in 513 cases NGU. 方法 用荧光定量多聚酶链式反应(FO-PCR)技术对513例非淋患者进行了UU DNA和CT DNA定量检测。
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Methods Realtime fluorescent quantitative RT-PCR was used to detect the CK20 mRNA expression in the portal venous blood of 31 patients with colon carcer and 20 healthy volunteers. 方法采用实时荧光定量RT-PCR检测31例病理学确诊的结肠癌患者门静脉血和20例健康对照者外周血CK20mRNA的表达水平。
- Methods: Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect HCV-RNA both in serum and in PBMCs of 65 patients with hepatitis C. 方法：应用荧光定量聚合酶链反应(FQ-PCR)方法，对65例丙肝患者的血清和PBMC中HCV-RNA进行检测。
