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- H9N2亚型AIV基因组序列分析及用反向遗传技术产生多个H9N2和H5重组流感病毒Genomic Characterization of an Avian Influenza Virus of H9N2 Subtype and Generation of H9N2 Reassortants and Attenuated H5 Recombinants by Reverse Genetics
- 将H9N2亚型AIV增殖,经差速离心和蔗糖密度梯度离心提纯后,灭活,免疫SPF鸡,制备高免血清并提纯免疫球蛋白G(IgG)。 以此制备疫苗免疫家兔,制备高免血清并提纯其中的IgG。High titer serum and immunoglobulin G (IgG) were prepared from specific pathogen free (SPF) chickens immunized with inactivated AIV subtype H9N2. Rabbits were immunized with this chicken anti-H9N2-AIV IgG and high titer serum were collected.
- H9N2亚型AIVH9N2 subtype avian influenza virus
- 因此,我们构建了单表达H5N1亚型AIV血凝素(HA)基因的重组病毒、双表达H5N1亚型AIV的HA和神经氨酸酶(NA)基因的重组病毒及双表达H5N1和H9N2亚型AIV的血凝素基因(HA)的重组病毒。The purpose of this study isdevelope better recombinant FPV vaccines against H5 and/or H9 subtype AI, in terms ofthe ability to resist the interference of maternal antibodies(MAbs). We constructedrecombinant viruses expressing HA gene of H5 or H9 subtype and coexpressing HA-NAgenes of H5N1 subtype ATV or two HA genes of H5 and H9 subtype AIVs with twodifferent FPV insertion vectors and two different wild type FPVs.
- 以A/African Starling/983/79(H7N1)毒株为模板，人工合成了H7亚型AIV的HA全基因序列，并将其克隆于pMD18-T载体。H7N1 AIV HA gene was synthesized from A/African starling/983/79(H7N7) cDNA template.
- H9N2亚型H9N2 subtype
- H9N2亚型禽流感AIV H9N2
- H9亚型AIVmildly pathogenic avian influenza virus
- H5N1亚型AIVH5N1 subtype avian influenza virus
- H9N2亚型流感病毒H9N2 influenza virus
- H9N2亚型禽流感病毒H9N2 subtype avian influenza virus
- 抗原特异性试验证明,重组HA1蛋白与鸡H5、H9亚型AIV抗血清不发生交叉反应。An antigen specific test showed that the recombinant did not have any cross-reaction with chicken H5 and H9 anti-sera.
- 禽流感病毒H9N2亚型Avian Influenza Virus H9N2
- H9N2亚型禽流感病毒TJ1株H9N2 subtype Avian influenza virus TJ1 strain
- 甲3亚型Subtype A3
- ζ亚型ζ isoform
- 分子亚型Subtype
- 亚型分析Subtype analysis
- 亚型鉴定Subtype identification
- 亚型学名Subtype scientific name
