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- Keywords BVDV;Full-length gene clone;Long overlapped PCR;Infectifious cDNA clone;Transient expression;DNA vaccine; 全基因克隆;长片段融合PCR;感染性克隆;真核表达;核酸疫苗;
- The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques. 采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
- By designing four primers for an overlapping PCR, we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase(CAT). 通过设计4个引物进行重叠（overlapping）PCR，由此克隆了人肿瘤坏死因子受体I死亡域（deathdomain）与氯霉素乙酰转移酶（CAT）的融合蛋白基因（DdLcat）。
- Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T. 方法:应用Overlap PCR的方法制备Protein A的Z结构域(Protein A-Z),克隆于pMD-18T载体构建PA-Z/pMD-18T重组质粒。
- Bags will no longer overlap the Bank UI when the game window is resized. 游戏窗口大小调整后物品袋不会和银行界面重叠了。
- The equity gap exists because two well-trawled sources of finance no longer overlap. 股权空隙的出现，源于上述两大对新兴企业提供巨大支持的经融资源不再重叠。
- Keywords Immunotoxin;Overlapping PCR;Prokaryotic expression; 免疫毒素;链重叠PCR;原核表达;
- There was a long lag in forwarding mail to us. 转寄给我们的邮件晚了很久才拿到。
- You can keep my book as long as you like. 我的书你要借多久都随你的便。
- Fruit juices ferment if they are kept a long time. 果汁若是放置很久，就会发酵。
- The beggar spun a long yarn about his misfortunes. 那个乞丐编造了一大篇自己如何遭遇不幸的故事。
- How long will our food supplies hold out? 我们的储粮还能维持多久?
- It was a long trudge up the hill. 那段上山的路很难走。
- Mr Jones has a long history of heart trouble. 琼斯先生有长期的心脏病史。
- Keywords Lagurus lagurus;zona pellucida 3;overlap PCR;codon optimization;prokaryotic expression; 草原兔尾鼠;卵透明带3;重叠PCR;密码子优化;原核表达;
- Cream cakes are no long a luxury. 奶油蛋糕再也不是奢侈品了。
- It took a long while to do the work. 做这个工作花了许多时间。
- Between ourselves, that young man's hair is much too long. 就咱俩之间说说，那小伙子的头发实在是太长了。
- It may take a long time to find a solution to the problem. 也许要花很长时间才能找到解决这个问题的办法。
- It was very thoughtless of you to keep her waiting so long. 你让她等那么久也太不体谅人了。
