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The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
Objective To investigate the amelogenin expression of recombinant plasmids PsecTaq2A-AMG in COS1-cell line. 目的研究人釉原蛋白重组质粒PsecTaq2A-AMG在COS-1细胞系中的表达,为基因工程制备釉原蛋白奠定基础。
The recombinant plasmids showed an adjuvant effect,and CpG2 was the best adjuvant among the plasmids. 发现CpGDNA重组质粒中无论插入CpG基序多或少都能增强小鼠抗囊尾蚴总抗体和IgG2a抗体亚类含量,都具有免疫佐剂效应,但其免疫强度有差别,其中CpG2的佐剂效应最强;
Methods pBP-wt-PTEN recombinant plasmids were transfected into ZR-75-1 cells by Lipofectamine. 方法利用脂质体介导法将重组质粒pBP-wt-PTEN导入人乳腺癌细胞ZR-75-1中,嘌呤霉素筛选稳定表达细胞株并扩增培养;
Results The structures of recombinant plasmids were comfinaed by PCR and sequencing. 结果重组体经PCR和测序证实准确连接。
The recombinant plasmid with the hammerhead ribozymegene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD. 将1.;7kb安普霉素抗性基因aprA插入aveD的NruI位点，得pDA1118。
Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast. 人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein. 目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。
Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line. 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。
A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique. 利用基因重组技术，构建含IL6基因的重组质粒pUC－IL6。
The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis. 反义TIMP 1表达质粒对肝纤维化有一定的逆转作用
The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
The recombinant plasmid pPGVT3 derived from the vector pUC4 was unstable in the E. 从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃培养得不到转化子。
The recombinant plasmid was transformed into E. coli BL21(DE3), and E. coli BL21(DE3)/pET-LG3 was induced by IPTG. 用IPTG诱导,LG3蛋白在大肠杆菌BL21(DE3)中得到了表达,并经Ni-NTA层析柱获得纯化。
The recombinant plasmids pQE-30/BCOADC-E2, pQE-30/PDC-E2, pQE-30/OGDC-E2 and pQE-30/BPO were transformed into plasmid E. 7例M2阳性的血清中，抗BCOADC-E2抗体、抗PDC-E2抗体、抗OGDC-E2抗体单独阳性的各一例，抗BCOADC-E2抗体、抗PDC-E2抗体同时阳性的3例，抗PDC-E2抗体、抗OGDC-E2抗体同时阳性的1例。
Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps. 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;
Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
The Arresten gene was screening successfully,and the recombinant plasmid pBV220-Arr was constructed successful. 成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达。