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- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- The results of reverse spot hybridization for both duck hepatitis B virus DNA (DHBV DNA) and special DNA polymerase were compared with those by dot blot for DHBV DNA and electromicroscopy for DHBV particles. 本文将逆向斑点杂交同时检测鸭乙型肝炎病毒DNA(DHBV DNA)及其特异DNA多聚酶(DNAp)的结果,与斑点杂交检测DHBV DNA和电镜检测DHBV颗粒的结果作了比较。
- We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR. PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来，既利用了PCR技术灵敏度高的优点，又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点，在实验室和现场的初步应用中都取得了较好的结果。
- Apply the PCRDIG probe to quickly detect the SRY gene by DNA dot blotting. PCR-DIG探针斑点杂交法快速检测SRY基因
- PCR-flow through reverse dot blot PCR-流过式反向斑点杂交
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- Using the 900bp fragment labeled by DIG as probe, dot blotting analysis demonstrated Sox9 was obviously present in the allotetraploid fish genome. 用地高辛标记的900bp片段做探针，斑点印迹结果显示，Sox9基因明显存在于四倍体鱼基因组中。
- The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting. pcDNA3.;1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞，用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达；
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特异性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Identification of Differential Expression ESTs in Sheep Ovarian Tissue by Reverse Northern Blotting 对绵羊卵巢组织差异表达ESTs的鉴定
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- The results of Dot Blotting and Western Blotting showed that the expressed hBMP4 and hBMP7 were identified separately by anti-BMP4 antibody and anti-BMP7 antibody. 经斑点印迹和蛋白质印迹证实了表达产物含有hBMP4与hBMP7，均具有良好的抗原性和特异性。
