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- Sanger dideoxy DNA sequencing 脱氧测序法
- The PCR products were purified and sequenced by the methods of Sanger dideoxy. 方法用一对引物进行PCR扩增,扩增产物纯化后用双脱氧终止法测序。
- The kinetoplast DNA of Trypanosoma evansi isolates, XJCA strain, AHB strain and YNB strain, were amplified by PCR, then the DNA sequences of the PCR products were analysed by the dideoxy chain termination method of Sanger. 对伊氏锥虫新疆骆驼株（XJCA）、安徽水牛株（AHB）和云南水牛株（YNB）的动基体DNA进行PCR扩增后，用Sanger双脱氧核糖核酸法测定扩增产物的序列。
- Methods The DNA sequencing of LPL gene of the patients was performed with the dideoxy method based on the polymerase chain reaction amplification using the genomic DNA as a template. 方法以基因组DNA为模板，借助聚合酶链反应扩增产物，用双脱氧末端终止法，对患者LPL的DNA序列进行检测。
- Method: PCR amplification,DNA sequencing and cladistic analysis. 方法:PCR扩增、DNA序列测定、分支分析。
- HBeBP4A gene was successfully amplified and identified by DNA sequencing. 成功扩增出HBeBP4A基因,测序结果符合GenBank报告序列。
- Laboratory diagnosis of Hgb Hekinan using HPLC and DNA sequencing methods? 施浤彰(Hung-Chang Shih);施木青(Mu-Chin Shih);张煜昌(Yu-Chang Chang);林惠茹(Hui-Ju Lin);林彩秀(Tsai-Hsiu Lin);彭庆添(Ching-Tien Peng);张天杰(Tien-Jye Chang);张建国(Jan-Gowth Chang)*?
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- New primers were designed for hot - start PCR reaction according to the results of DNA sequencing. 根据测序结果设计新的引物以进行热启动PCR反应；
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- A deletion mutation with frameshift of neurofibromatosis type 2 gene was detected by DNA sequencing. 以DNA序列分析出，在第二型纤维细胞瘤基因上发生染色体缺失造成移码突变。
- Sanger dideoxy sequencing 桑格双脱氧测序法
- Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT－PCR技术从人B淋巴瘤细胞系Raji中克隆到B7－1（CD80）cDNA，并经测序证实。
- Methods STK 11 germline mutation was analysed by DNA sequencing in 18 unrelation patients with PJS. 方法 用 DNA直接测序方法 ,对 18个家系的 PJ综合征患者 STK1 1 基因 9个外显子进行研究。
- Detection of keratin 5 gene point mutation in a family with Weber Cockayne EBS by PCR and direct DNA sequencing. PCR-DNA直接测序检测1例单纯型大疱性表皮松解症Weber-Cockayne亚型(WC-EBS)患者角蛋白K5基因点突变
- The more varied the organisms, the more varied the DNA sequences. 生物变化越多，DNA序列的变化也就越多。
- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法：采用多聚酶链反应（PCR），变性梯度凝胶电泳（DGGE）和DNA测序等方法对上海地区血友病甲基因突变进行检测。
- The more varied the organisms,the more varied the DNA sequences. 生物变化越多，DNA序列的变化也就越多。
- DNA microarray has been applied to DNA sequencing,pharmaceuticals analysis,gene expression and so on. DNA微集阵列已广泛用于DNA测序、药物分析、基因表达等研究领域。
