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- Using Specific Primer pair to Detect Bursaphelenchus xylophilus 应用特异引物组合检测松材线虫
- Primer pairs C1/C2, D1/D2 and G1/G2 amplified different fragments from several genomic DNA samples. Further study should be done to improved PCR conditions or search for other specific primers for the detection of Cur. 引物C1/C2、D1/D2和G1/G2的扩增结果:来自于Cur.
- Specific primer pair 特异引物组合
- A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E. 根据已知的cry2Ab基因ORF序列,设计合成了一对特异性引物,PCR扩增出cry2Ab基因全长,并直接与pUCm-T载体相连。
- One marker, named as Nsp4, was obtained with the primer pair A3B6, whose genetic distance to SSN resistance gene was 8.73 cM. 引物组合A_3B_6找到了1个标记;命名为Nsp4;与抗茎线虫基因的遗传距离为8.;73cM。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物,扩增出运动蛋白基因的整个开放阅读框(ORF)。
- RC1(3+4), ALC(5)141 and LC1(5)57-4 deriving from 9248-6 lost one band based on the AFLP primer pair, 92S08/92G08. 4. The Pot2-PCR based fingerprints showed that lineage closely related to the cultivar with given R-genes. 92S08/92G08 引物对揭示3 个来自9248-6的菌株与其亲本菌株相比,缺失1 条带。
- Methods HLA-B* gene polymorphism in 31 patients with DCM and 29 normal control subjects was analyzed using polymerase chain reaction-sequence specific primer(PCR-SSP) technique. 方法采用聚合酶链反应和顺序特异性引物(PCR-SSP)基因多态性分析方法,对31例扩张型心肌病患者及29例无血缘关系健康人的HLA-B*各等位基因及亚基因进行检测分析。
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因(apxI,apxII,apxIII,apxIV)。
- A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence. 根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
- By using these two primer pairs and two times of PCR protocols, Cur. 所以使用这两对引物进行两次PCR反应可将目标菌Cur.
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同,需通过DGGE检测证明是一种DNA序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素(LT)的基因序列设计引物,用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
- A chitinase A gene (chiA) was successfully amplified by PCR using a pair of specific primers from Serratia marcescens Q901, and cloned into pUC19 vector. 用设计的特异性引物和PCR技术,成功地从粘质沙雷氏菌(Serratia marcescens)Q901基因组DNA中扩增并克隆几丁质酶A基因(chiA)序列(GenBank登录号:AY433954)。
- Using primer pairs F1/F2, an amplified fragment was obtained from each of the genomic DNA of Cur. 引物F1/F2扩增结果:F1/F2可以从Cur.
- I bought a pair of tan leather shoes the other day. 前几天我买了双棕褐色的皮鞋。
- This test developed a pair specific primers of P4.1 and P4.2.Using the specific primers P4.1 and P4.2 in the PCR,only 6 kava materials amplified the 562bp specific band,the others were not any amplified. 研究开发了1对卡瓦胡椒特异SCAR引物P4.;1和P4
- Leishmania DNA was detected in 33%(33/100)and 30%(30/100)human blood samples by RV1-RV2 and K13A-K13B primer pairs respectively. 这两对引物对100份无症状居民血的阳性检出率分别为33%25(33/100)和30%25(30/100)。
- AFLP markers are exploited in map construction. 140 ploymorphic bands are generated by 17 primer pairs in EcoRI/Msel and Pstl/Msel eynzem combinations. 利用EcoRI/MseI和PstI/MseI两种酶切组合,分别筛选了64种引物组合,9对来自EcoRI/MseI酶切组合的引物和8对来自PstI/MseI酶切组合的引物进行了群体分析。
- According to the relevant nucleotide sequence from GenBank, a pair of specific primers was designed to amplify the partial fragment of ahyR gene from Aeromonas hydrophila J-l strain by PCR. 根据GenBank公布的嗜水气单胞菌转录调控蛋白ahyR基因的序列设计一对特异性引物,利用PCR方法扩增Ah J-1株ahyR基因的部分片段(656 bp)。