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- The fragment containing UBE gene was inserted into pGEX-4T-2 expressive vector, and induced by IPTG. Molecular weight of ubiquitin fusion protein was about 40 kD by checking with SDS polyacrylamide gel electrophoresis. 将烟夜蛾的UBE基因克隆到原核表达载体pGEX-4T-2上,经IPTG诱导,SDS-PAGE电泳检测出约40kD的融合蛋白。
- Ubiquitin fusion protein 泛素融合蛋白
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin. 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上，转化至大肠杆菌BL21（DE3）中，用IPTG进行诱导表达，用异源泛素单克隆抗体进行Western blot检测，证明了原核表达蛋白是目的蛋白。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- The titer of antiserum was detected by double agar immunodiffusion with GST-PI fusion protein. 并用双向免疫扩散法鉴定家兔多价抗血清的效价。
- EGF-Ang fusion protein had definitely cytotoxicity to Hep2 cells which over expressed EGFR in vitro. 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用。
- Recombinant TrxA/PEB1 fusion protein could be a valuable candidate for CJ vaccine with great applied value. （4）PEB1融合蛋白作为CJ的亚单位侯选基因工程疫苗不仅可行，而且有良好应用前景。
- Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.coli. 结果:成功克隆了CAT的基因片段,并在大肠杆菌中得到其融合蛋白的表达。
- A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. 利用基因工程技术在大肠杆菌表达成功人白细胞介素2（IL－2）和人血管生成素（Angiogenin）的融合蛋白（IL－2－Ang）。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
- Cloning and Characterization of a Ubiquitin Fusion Degradation Protein Gene in Wheat 小麦泛素融合降解蛋白基因的克隆及特征分析
- Western blot proved that the recombinant protein is the fusion protein of APE/Ref|1. Western印迹证明重组蛋白为APE ref 1融合蛋白 .
- These fusion proteins were used to immunize rabbits to raise antisera. 这些表达产物用于免疫动物产生多克隆抗血清。
- Western-blot analysis with sera against CAV showed the fusion protein had good specificity. 在Western-blot免疫印迹分析中，3个融合蛋白与CAV阳性血清均能发生特异性反应，表明它们都具有良好的反应性。
- The expression vector of PVS CP gene is constructed, a 58kDa fusion protein product is expressed in E. 构建了含PVS CP基因的融合蛋白原核表达载体，并在大肠杆菌(E.;coli
