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- 10 Zhang JZ,Xu XM.Ma WF,et a1.A rapid reverse dot blot assay [张基增.;徐湘民
- Keywords Prevotella nigrescens;human hemoglobin;iron acquisition;dot blot assay; 黑色普氏菌;人血红蛋白;铁摄取;斑点印迹法;
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- The results from enzyme-linked assay and dot blot experiment show that an enrichment pool was obtained after four rounds selection, which can specifically be used to recognize ricin. 酶联免疫和斑点杂交实验结果表明，仅经4轮筛选即可获得特异识别蓖麻毒素蛋白的寡核苷酸适配子。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- The expression of fascin was tested by Western blot assay in 26 pairs of ESCC and its cancer-adjacent normal tissues. 在癌旁正常组织中fascin几乎不表达或表达量很低，而在食管癌组织中fascin表达水平明显增高。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
- Western?blot assay demonstrates that McAb 4G4,2H4 and FA4 belong to different lipopolysaccharide (LPS) fraction of Aeromonas hydrophila. Western blot结果表明,单抗4G4、2H4、FA4所针对的抗原是菌体脂多糖(LPS),且它们所识别的LPS抗原表位不同。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特异性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- We have demonstrated the constitutive polypeptides (PP) of Dane parti-cles employing SDS-PAGE Western Blot assay, investigated the antibody res-ponse ability to those PP in the HBV infected subjects in comparison withother HBV serum markers. 采用PAGE-Western Blot技术对血源性Dane颗粒的多肽组份进行了分析。 并结合HBV感染的其它血清学指标,初步探讨了HBV感染者对这些多肽的抗体应答情况及意义。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- A total of 195 sera from France and Ivory Coast were tested. A good correlation was obtained, all 76 sera with HIV-l antibody by Western blot assay were also positive by PA test and ELISA. 共检测了195份来自法国和非洲象牙海岸的血清,凡是蛋白印迹法阳性的血清,明胶颗粒凝试验都是阳性。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Methods: Expression of COX-2 mRNA and its protein in the patients with CML was detected by reverse transcript polymerase chain reaction (RT-PCR) and western blot assay(westernblot). 方法:通过逆转录聚合酶链反应(RT-PCR)和蛋白免疫印迹(Westernblot)分别检测63例慢性粒细胞白血病(CML)患者血细胞中COX-2的mRNA和蛋白的表达;
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛随机引物标记试剂盒对该片段进行标记后,对不同水分和硫营养条件下提取的小麦根系总RNA 进行斑点杂交。
