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Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 细胞核载体PB-adw、PBG-adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经PCR、PCR-Southern、southern斑点杂交分析证实，adw基因已整合到莴苣基因组中。
DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA点杂交检测表明73.;4%25的抗性植株同时含有GNA基因和hpt基因;Southern杂交分析进一步证实了GNA基因在转基因水稻基因组中的整合
Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通过对有绿色荧光的烟草、矮牵牛进行dot blot、southern blot分析，都获得阳性杂交结果，证实gfp基因已整合到植物基因组中并与绿色荧光观察结果一致。
Using the 900bp fragment labeled by DIG as probe, dot blotting analysis demonstrated Sox9 was obviously present in the allotetraploid fish genome. 用地高辛标记的900bp片段做探针，斑点印迹结果显示，Sox9基因明显存在于四倍体鱼基因组中。
ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot. 方法：利用基因工程技术合成马来丝虫寡核苷酸片段，经32P标记后作为探针，以斑点杂交法检测蚊体内马来丝虫幼虫。
The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
Cell culture in vitro,ABC ELISA,RNA dot blot and atom absorption spectrum analysis were used to study biological effects of low concentration of iron citrate (Fe cit) on the expression of transferrin receptor (TfR) in healthy human peripheral lymphocytes. 为了研究铁缺乏的生物学作用，采用体外细胞培养、ABC－ELISA法、RNA斑点杂交和原子吸收光谱分析等技术方法，观察了低浓度柠檬酸铁（Fe－cit）对健康人外周血淋巴细胞转铁蛋白受体（TfR）及其基因表达的影响。
Identified by individual plant testing , analysis of RT-PCR and dot blot ,this fragment was only found in CMS cauliflower knxd612.Analysis of the sequence indicated it was high homologous(98%) with orf138 of Ogura CMS radish. 单株检测,RT PCR分析,斑点杂交鉴定,确定此片段为花椰菜细胞质雄性不育系 knxd612 所特有。序列分析表明该片段与Ogura型胞质不育萝卜,不育相关开放读码框orf138的同源性高达98%25。
Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
Students practise using the PQRS analysis technique with assignment titles. 学生练习使用PQRS题目分析技术来分析题目。
Method:By using the SABC immunohistochemical and the image analysis technique. 方法:采用SABC免疫组织化学染色方法,并结合图象分析技术。
It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 荧光分光光度计检测P2X7受体介导的胞内钙离子浓度变化。