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- The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. 同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
- Aim:To study the dynamic expression of green fluorescent protein (GFP) and enhanced green fluorescent protein (EGFP) on C6 glioblastoma cells in vivo. 目的:研究绿色荧光蛋白报道基因体外转染C6胶质瘤细胞系的动态表达。
- Objective: To express and purify fusion protein from angiogenin(ANG) and enhanced green fluorescent protein (EGFP) to further study the function of angiogenin. 目的:表达并纯化血管生成素(ANG)与增强型绿色荧光蛋白(EGFP)融合蛋白,以进一步探讨血管生成素的生物学功能。
- Objective In order to observe the infiltration and metastasis of the tumor,establish a high-metastatic lung cancer cell line expressing enhanced green fluorescent protein(EGFP). 目的建立能稳定高表达增强型绿色荧光蛋白(EGFP)的肺癌细胞株,为研究肺癌的浸润与转移机制打下基础。
- AIM: To investigate an effective transfection method of non-viral vectors and the expression of enhanced green fluorescent protein (EGFP) in the transfected immortalized neural progenitor cells (INPC). 目的:寻找能高效转染永生化神经前体细胞(IN-PC)的非病毒载体;观察转染后增强型绿色荧光蛋白(EGFP)的表达.
- AIM: To observe the enhanced green fluorescent protein (EGFP) gene expression and cytotoxicity in human adipose derived adult stem cells (hADSCs) by pEGFP-N1, Ad5-EGFP and rAAV-2/1-EGFP, and investigate the suitable gene-transferred vehicle. 目的:观察脂质体介导质粒pEGFP-N1、重组腺病毒Ad5-EGFP、重组腺相关病毒rAAV-2/1-EGFP基因转染人脂肪来源成体干细胞后增强型绿色荧光蛋白的表达及细胞毒性,探讨适用于脂肪来源的成体干细胞的转基因方法。
- MSCs were transfected with enhanced green fluorescent protein (EGFP) gene under the mediation of LipofectAMINE, and G418 resistance was adopted for screen, so as to observe the transfection efficiency. 在脂质体介导下,将增强的绿色荧光蛋白基因导入骨髓间充质干细胞,然后通过G418抗性筛选,观察转染效率。
- Methods: The transient expression system of COS7 was constructed by using pcDNA3.1-sEGFR501 expression vector and the process of Calcium-phosphate co-precipitation transfection was optimized with Enhanced Green Fluorescent Protein (EGFP) as a reporter. 方法：以增强型绿色荧光蛋白(EGFP)基因为报告基因，优化磷酸钙共沉淀转染COS7的实验参数； 以pcDNA3.;1-sEGFR501和COS7组建瞬时表达系统。
- Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with enhanced green fluorescent protein (EGFP) as report gene and to observe its expression in PC12 cells. 目的构建以增强型绿色荧光蛋白(EGFP)作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体,并转染PC12细胞,观察外源基因的表达情况。
- The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer. 本研究是第一次报道在体细胞克隆和胚胎移植后成功应用嘌呤霉素标记获得了转强化型绿色荧光蛋白（EGFP）基因的小猪。
- Methods NSCs were primarily cultured and labeled with the enhanced green fluorescent protein(EGFP),and then were transplanted in hippocampal CA3 of adult chronic epileptic rats treated by KA. 方法将增强型绿色荧光(EGFP)标记的原代培养NSC移植到慢性癫疒间鼠的海马CA3区。
- The study observed the transfection efficiency of recombinant adeno-associated virus with enhanced green fluorescent protein transduction to rat bone marrow mesenchymal stem cells in vitro. 如果进一步在基因水平上分析转染后的骨髓间充质干细胞有哪些生物学特性，如外源基因的表达、细胞凋亡的变化等，实验结果会更加完善和系统。
- Enhanced green fluorescent protein (EGFP) gene 增强型绿色荧光蛋白基因
- Enhanced green fluorescent protein C3 增强型绿色荧光蛋白C3
- Enhanced green fluorescent protein gene 增强绿色荧光蛋白基因
- Enhanced green fluorescent protein(EGFP) EGFP
- enhanced green fluorescent protein 增强型绿色荧光蛋白
- enhanced green fluorescent protein (pEGFP) 绿色荧光蛋白
- enhanced green fluorescent protein(EGFP ) 增强型绿色荧光蛋白
- enhanced green fluorescent protein(pEGFP) 绿色荧光蛋白
