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- The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
- Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. 蛋白胨是一种酶消化的蛋白质用来培育非挑剔的有机体,生产低级别的增长。
- Method:The olfactory receptor neurons could be isolated from olfactory mucous of Kunming mouse using enzymatic digestion and mechanical isolation. 方法利用酶消化结合机械吹打方法,急性分离昆明小鼠嗅粘膜的嗅感觉神经细胞。
- The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
- The study on enzymatic digestion of collagen in recent years were described. The application prospect of the collagen polypeptide was analysed. 阐述了近年来国内外关于酶法水解胶原蛋白所做的研究工作,并对胶原多肽的应用前景进行了分析。
- Chemists at the University at Buffalo found that the electric charge on these nanoparticles held and compacted the DNA, protecting it from enzymatic digestion in cells. 美国纽约州立大学水牛城分校的化学家发现,这些奈米微粒上的电荷会紧密包夹DNA,保护这些DNA免受细胞中酵素的分解。
- The middle ear effusion signifcantly inhibits DNase 1.Conclusions Middle ear effusion provides an inhibition of the enzymatic digestion of purified DNA.Thus any DNA fou... 结果中耳分泌液和DNA的粘度是稳定的,中耳分泌液不降解DNA,DNA酶迅速消化DNA,中耳分泌液显著抑制DNA酶消化DNA的活性。
- The enzymatic digest of bovine serum albumin(BSA),used as a model protein, was directly analyzed by reversed phase high performance liquid chromatography (RP HPLC ) /electrospray tandem mass spectrometry (ESI MS/MS). 利用反相高效液相色谱 (RP HPLC)和电喷雾串联质谱 (ESI MS MS)联用技术直接对模式蛋白分子 (牛血清白蛋白 ;BSA)的胰蛋白酶酶解产物进行分离和测定 .
- Isolation of adult DUM neurons was performed under sterile conditions using enzymatic digestion and mechanical dissociation of the median parts of the TAG from the nerve cord of the cockroach Periplaneta Americana. 使用酶消化和机械分离出成年美洲蜚蠊的腹部末端神经节(TAG),然后在无菌条件下分离DUM神经元细胞。
- METHODS: The glioma cells from 12 glioma patients were cultured primarily by the tissue pieces culture method and enzymatic digestion culture method, and the effects of culture were compared. 方法:分别采用组织块培养法和酶消化法对12例人脑胶质瘤细胞进行原代培养,比较两种方法的培养效果;
- The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
- Cationic colloidal gold (CCG) probe and various enzymes digestion on CCG labelling were taken for the study. 采用阳离子胶体金(CCG)探针和系列酶消化包埋后标记法观测。
- Nucleic acid enzymatic digestion method 核酸酶消化法
- Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
- Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法,但是也都有各自的缺点。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果:酶切鉴定及测序结果提示表达产物正确;
- Protoplast The protoplasm and plasma membrane of a cell after removal of the cell wall, where present. This can be achieved by physical means or by enzymic digestion. 原生质体:指去掉细胞壁以后的细胞原生质以及质膜。可用物理方法或酶消化得到。
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体,EcoR I酶切鉴定。
- Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶/DNA酶消化法进行消化,经光镜和电镜观察。