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- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- Nonspecific and specific phagocytic activities of macrophages with heterogeneous fluorescent staining were observed using starch polysaccharide and immune activators (diphtheria toxoid and BCG) to induce and activate mouse peritoneal macrophages. 利用淀粉多糖和免疫促进剂(白喉类毒素和卡介苗)诱导和活化小鼠腹腔巨噬细胞,观察了四种异质性荧光染色的巨噬细胞非特异性和特异性吞噬活性。
- Immunoreactivities for Fas, FasL and caspase - 3 in-creased considerably in the cervical carcinoma tissue. Dual positive fluorescent staining for ap-optosis and caspase - 3 or Fas was appeared in some cells. 癌组织中,Fas、FasL、caspase-3蛋白阳性细胞明显增多,双重荧光染色显示,部分细胞Fas及cspase-3蛋白与凋亡细胞同时阳性染色。
- MTT method was employed to detect the proliferation of cells,while the adhesive ability was investigated by stereology. In the meantime,the cell integrity and the interface between biomaterial and cells were observed by fluorescent staining and SEM. 采用MTT法检测细胞增殖活性,体视学方法检测细胞粘附能力,荧光双染法检测细胞完整性,扫描电镜观察细胞-材料界面。
- Methods For 23 cases (38 eyes) diagnosed of keratoconjunctivitis sicca, conjunctival impression cells were examined by using acridine orange fluorescent staining, the results being evaluated and graded by Tseng Classification. 方法选自我院眼科门诊已确诊的干燥性角结膜炎患者23例(38眼),用丫啶橙荧光染色结膜印迹细胞检查法进行检测,并用结膜印迹细胞检查结果评价标准进行评价,按Tseng分类法进行分级。
- Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated. 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);
- Aim To evaluate the functional condition of corneal cells and to detect the degree of keratoconjunctivitis sicca using conjunctival impression cell test with acridine orange fluorescent staining. 目的应用丫啶橙荧光染色法进行结膜印迹细胞学检查,通过判断结膜细胞功能状态,对干燥性角结膜炎患者的眼部干燥程度进行定量检测,为临床的治疗提供实验室依据。
- Methods Twenty three patients with serious blepharitis were given slit-lamp examination, tear break-up determination, Schemer 1 test and corneal fluorescent staining before and after drug therapy. 方法对23例严重睑缘炎患者进行详细裂隙灯检查、泪膜破裂时间、泪液分泌试验测定及角膜上皮荧光素染色。
- The tumor cells treated with various chemotherapeutic drugs were stained with dual fluorescent staining. 细胞标记采用双重萤光染色。
- Rh123/PI dual fluorescent staining Rh123/PI双染法
- Morphological change of macrophages observed with transmission electronmicroscopy and fluorescence staining. 然后利用透射电镜和荧光染色观察凋亡巨噬细胞的形态学特征;
- acridine orange fluorescent staining 吖啶橙荧光染色法
- differential fluorescent staining 鉴别性荧光染色法
- auramine-rhodamine fluorescent staining 金胺-罗丹明荧光染色
- The new device is the first 3-D microscope that can use both traditional and fluorescent stains, Seibel said. 该仪器是第一个能同时使用传统和荧光染色的立体显微镜,赛贝尔说。
- Furthermore, the induction of apoptosis by CSE in HepG2 cells was observed with AO/EB fluorescence stain. 0mg/mL赤参水提物处理HepG2细胞24h后,细胞荧光染色出现了明显的凋亡细胞;
- He vainly tried to mark the defacing stain out. 他试图除去有损外观的污迹,但没有成功。
- I put the shirt in soapy water to soak out the stain. 我将衬衫浸在肥皂水里以去除污渍。
- I scrubbed hard at the stain to see if I could get it off. 我使劲擦这个污迹,看能不能将它去掉。
- The apoptosis of suspension cultured cells of Viola diffusa Ging. which had been induced by Ce(NH4)2(NO3)6 were detected by DNA Laddering and DAPI fluorescence stain methods. 用DNA Laddering和DAPI荧光检测法对Ce(NH4)2(NO3)6诱导的蔓茎堇菜(Viola diffusa Ging.;)