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Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps. 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;
Optimization of the Amplified Conditions for Human Apo E Gene Fragment by Uniform Design. 用均匀设计优化apoE基因的PCR扩增方案
Methods:We have amplified a gene fragment encoding the BCD domain of ER and overproduced MS?2-ER fusion protein in E. 方法：扩增编码ERBCD区的基因，在大肠杆菌中表达MS2－ER融合蛋白。
AIM:To locate gene fragment BU581985 in fetal rabbit skin by using in situ hyb ridization(ISH). 目的:利用原位杂交技术将BU581985基因片段在胎兔皮肤定位。
A gene fragment encoding three copies of proinsulin C peptide was synthesized and expressed in E. 合成了胰岛素原C肽三聚体的基因,并在大肠杆菌中得到高效表达。
Herpes simplex virus glycoprotien D gene was amplified from HSV 2 Sav strain. The D gene fragment was cloned into M13mp18 and M13mp19 and sequenced. 从单纯疱疹病毒2型（HSV?2）Sav株中扩增糖蛋白D（gD）基因646bp片段，克隆入M13mp18、M13mp19测序载体。
Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
Digesting vector MINV-IRES-mB7-2 by enzyme MuntI and BamHI to obtain IRES-mB7-2 gene fragment with ambly end and BamHI sticking end. 2MunI酶切载体MINV-IRES-mB7-2,末端钝化,然后BamHI再酶切,得到含钝末端和BamHI粘末端的IRES-mB7-2基因片段;
Methods: CAT gene fragment was cloned via PCR technigue,which was later exprecsed in Eschen chia coli with protein reconstruction technigue. 方法:通过PCR技术克隆CAT的基因片段并通过蛋白重组融合表达技术使其在大肠杆菌中表达。
The TK gene fragment (1.36Kb) of the six APV strains was amplified by polymerase chain reactioin (PCR) using the primers H1+ and H2-. 通过PCR方法用自行设计的一对引物H1+\\H2-扩增出了四个鸽痘病毒分离株、鸡痘病毒疫苗株和鸽痘病毒疫苗株的TK基因片段，长度约为1.;36Kb。
Objective To construct eukaryotic expression vector carrying human Apoptosis-inducing factor(AIF) gene fragment,pcDNA3.1(+)/AIF,and research the gene expression and function. 目的构建人凋亡诱导因子(AIF)基因功能片段的真核表达载体pcDNA3.;1(+)AIF;并初步研究AIF在细胞凋亡中的作用。
Results 1.Both GAP-43 ORF gene fragment and the recombinant AAV-EGFP-GAP-43 plasmid were verified by enzyme digestion and DNA sequencing for correct reading frame and orientation. 结果:.;1
The paper summarizes the applications of DNA sequences (cpDNA, nrDNA and mtDNA) in bryophyte phylogenesis, and discusses the strategies of gene fragment selection. 本文综述了DNA序列（叶绿体基因组、核基因组和线粒体基因组）在苔藓分子系统学中的应用，探讨了基因片段的选择策略。
The gene fragment encoding the N-terminal part of Cbl protein (named as CblN) was amplified by PCR using pEFHACbl plasmid encoding human Cbl as templates. 含有人全长CblcDNA的质粒pEFHACbl作为模板扩增Cbl基因N-端(CblN);
Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T. 方法:应用Overlap PCR的方法制备Protein A的Z结构域(Protein A-Z),克隆于pMD-18T载体构建PA-Z/pMD-18T重组质粒。
Professor Kang said to Miriam, our researchers try to HIV virus into the gene fragment of vaccinia vaccine, expected to be to find an effective AIDS vaccine. 康来仪教授透露说，我国科研人员尝试将HIV病毒的基因片断整合入牛痘苗中，期望能藉此找到有效的艾滋病疫苗。
To study character of the NDV-F protein epitope in the animal immune response,we cloned and expressed a gene fragment of NDV-F partial protein,and examined its immune reactivity. 为研究NDV-F蛋白抗原表位在动物免疫应答中的作用特点,作者克隆和表达了NDV-F蛋白基因的部分片段,并检测其免疫反应性。
The nucleotides sequence homology and putative amino acid sequence homology of the gene fragment were 99%,compared with Bacillus amyloliquefaciens subtilisin DFE precursor and Bacillus sp.DJ-4,respectively. 该基因片段核苷酸序列与Bacillus amyloliquefaciens subtilisin DFE precursor有99%25的同源性;对应的氨基酸序列与Bacillussp.;DJ-4有99%25的同源性。
The pCVP21 was contructed by inserting the amplified VP2 gene fragment into expression vector pCYTEXP1, containing bacteriophage promoters P R and P L in tandem preceded by the cIts 857 repressor gene. 扩增产物经双酶切后插入含噬菌体P?RP?L双强启动子的pCYTEXP1表达质粒，构建了VP2基因克隆pCVP21，经Dot?ELISA筛选出4个VP2表达阳性克隆。