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- These Abs were used for biopanning of a phage-displayed random15- peptide library. 并以此为筛选配基,对噬菌体表面展示的随机15肽库进行亲和筛选。
- These Abs were used for biopanning of a phage-displayed random peptide library of6 amino acid residues. 以此对噬菌体表面展示的随机6肽库进行亲和筛选。
- Phage display random peptide library is a kind of polypeptide library developed by filamentous bacteriophages. 摘要噬菌体随机展示肽库是一类利用丝状噬菌体发展起来的多肽文库。
- Objective To select minotope of NMDAR1 by using phage displayed random peptide library. 目的利用噬菌体展示随机肽库技术,进行NMDAR1抗原表位研究。
- Objective To screen two MUC1 antigen mimic epitopes by phage display peptide library technology. 目的从噬菌体表面展示的随机12肽库中筛选出MUC1抗原的模拟表位。
- Purpose To synthesize a peptide library of thymopentin with D-amino acids substituted, and to determine its effect of lymphocytic proliferation. 摘要目的合成胸腺五肽D型氨基酸取代肽库，并测定对T淋巴细胞的免疫活性。
- Objective To study the immunoactivity of the antigenic mimic epitopes of Schistosoma japonicum screened from phage 12-mer peptide library. 目的研究从噬菌体12肽库中筛选得到的日本血吸虫抗原模拟表位的免疫学活性。
- Teabag method was used to synthesize the peptide library of thymopentin with D-amino acids substituted by solid phase peptide synthesis (SPPS). 利用组合化学技术,采用简便的茶袋法固相合成制备胸腺五肽D型氨基酸取代类似物肽库,共合成32条肽。
- By selecting suitable protein scaffold, display and screening methods, the rational and constrained random peptide library(RPL) can be constructed. 选择适宜的蛋白骨架和展示、筛选方法，可构建基于合理蛋白骨架结构的优化限制性随机肽库。
- Methods:Screening peptide library with EAN serum. Identified positive clone by ELISA and compared similarity between positive clone and LPS of Campylobacter jejuni. 方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。
- To identify native epitope of N protein, we constructed the ORF7 gene-targeted peptide library and screened by the MAb N3H2. After 3 rounds of biopanning, 3 clones were obtained. 为进一步研究表位结构;本研究构建了PRRSV ORF7基因特异性肽库;利用N3H2进行了3轮筛选;获得3个阳性噬菌体克隆.
- Objective To identify the B-cell epitopes of dengue virus type 2 (DEN2) envelope glycoprotein (E) from a random phage displayed peptide library (RPL) using a serotype-specific monoclonal antibody (McAb) of DEN2 NGC strain. 目的 用噬菌体展示肽库筛选登革 2型病毒 (DEN2 )E蛋白的抗原表位 ,并确定该抗原表位性质。
- In the present paper the application of DNA library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed. 本文综述了基因文库、噬菌体展示肽库及噬菌体抗体库技术在血清学诊断试剂研制中的应用及其各自的优点。
- Using mAb F3 as target, a 12-mer phage peptide library was bio-panned for 3 rounds, and the phages eluted from 3rd panning were characterized by sandwich ELISA, competition ELISA and DNA sequencing. 用夹心ELISA和竞争ELISA鉴定筛选克隆的结合特性;并进一步对阳性克隆进行序列测定和分析.;结果通过3轮生物淘选;能被抗体捕获的噬菌体克隆为21/22
- Phage-displayed peptide library technology is based on expressing recombinant random peptides on the surface of filamentous phage and selecting function peptides. 噬菌体展示技术是将随机多肽文库表达于丝状噬菌体的表面,筛选功能性多肽的一种生物技术。
- Phage Display Peptide Library Kit 噬菌体展示试剂盒
- Phage display peptide library aud its applications 噬菌体展示肽文库及其应用
- Here, the basic mechanism and the application of phage display random peptide libraries are briefly introduced. 本文简介此类文库的基本作用原理及应用。
- Keywords SARS-CoV;epitope;phage peptide library; SARS冠状病毒;表位;噬菌体肽库;
- Phage display 12-mer peptide library 噬菌体随机十二肽库
