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- In this paper, these novel protein isotope labeling methods and alternative protein expression systems are reviewed. 本文旨在对这些蛋白质标记表达新技术的方法及应用予以逐一介绍。
- Additionally, one of the most obious benefits of using plants as protein expression systems is the potential for scale up. 另外,利用植物作为蛋白质表达系统的一个最重要的优势是拥有扩大生产的潜力。
- Objective To establish a protein expression system using pyrG gene as a selection marker and Aspergillus niger (A.niger) as a host. 目的 建立以pyrG基因为选择标志 ,以黑曲霉菌为宿主菌的蛋白质表达系统。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留,因此成为蛋白切割工具的热点。
- Signal peptides' structures and functions, signal sequence traps and the proteins expressions in prokaryote and eukaryote expression systems were illustrated in the article. 就信号肽的结构和功能,信号肽的捕获方法及其在原核表达系统和真核表达系统中表达外源蛋白质的应用做一些介绍。
- cell-free protein expression system 无细胞蛋白合成
- protein expression systems 蛋白质表达系统
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- The protein expression of Bax was increased after DATS treatment. 免疫组化显示;经DATS处理后;Bax的表达增强(P<0.;05);Bcl-2表达未见明显改变。
- Pichia pastoris expression system is a recently developed system to efficiently express foreign protein. 摘要毕赤酵母表达系统是近年来发展起来的一种高效表达外源基因的表达系统。
- Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. 蛋白质表示: 一实际方法细节克隆DNA 或者RNA 模板在全部主要方面的表示在活体内和在试管内系统。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- The positive rate of COX-2 protein expression was 61.67% in NPC tissues. COX-2在NPC中总的阳性表达率为61.;67%25。
- The bacterium Escherichia coli is a widely used host for protein expression. 大肠杆菌被广泛应用于蛋白表达的宿主。
- This experiment successfully constructed high performance Gly-Gln polymers prokaryotic expression system, whose expression level achieved 40% of total bacterial protein. 本试验成功构建了Gly-Gln多聚体原核表达系统,该系统能够高效表达Gly-Gln多聚体蛋白,表达的目的蛋白占总蛋白的40%25左右。
- Green florescent protein allows the identification of protein expression. 萤光绿蛋白可以用作检测蛋白表达。
- Results: VEGF protein expression was detected in 32 of 57 human gliomas. 结果:在57例胶质瘤中32例观查到了VEGF蛋白表达。
- Pichia pastoris is one of the most used expression systems for the high level expression of heterologous proteins. 巴斯德毕赤酵母是目前应用最广泛的外源蛋白表达系统。
- Using recombinant plasmid pET28a(+)-GNLY/BL21(DE3)pLysS expression systems, the GNLY protein with biologic activity could be produced, and the strategy is available to prepare human GNLY in large quantity by gene engineering technique. 用含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS表达系统,可得到具有生物活性的重组颗粒溶素,为大批量生产提供了条件。
- Single-chain Fv has mature prokaryocyte expression system with high yield, but its stability is poor. 单链抗体有较成熟的原核表达系统,产量较高,但稳定性较差。