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- Objective To evaluate the telomeric restriction fragment in the progress of gastric tumorigenesis. 目的探讨不同胃粘膜病变端粒状态的变化规律。
- Rep-PCR and restriction fragment length polymorphism (RFLP) were used to analyze the genetic structure in a population of 143 Xanthomonas oryzae pv. 用Rep-PCR和RFLP两种方法分析了143个水稻白叶枯病Xanthomonas oryzae pv.
- Method 32 cases of hydatidiform mole were analyzed by DNA restriction fragment length polymorphism (RFLP, hybridized with the probe 33.15). 方法采用DNA限制性片段长度多态性分析的方法研究32例葡萄胎标本。
- Restriction Fragment Length Polymorphisms (RFLPs) between two rice varieties , Zhaiyeqing(Indica) and Jingxi17 (Japonica), were analyzed. 摘要 对窄叶青(籼稻)和北京17(粳稻)的RFLP进行了系统分析。
- Errors intrinsic to the testing systems, such as the inability to precisely measure DNA restriction fragment lengths, are well compensated for by interpretation guidelines. 鉴定系统本身固有的误差,如不能精确的测量DNA限制酵素片段长度,可以由解释规则来很好的弥补.
- As a codominant genetic marker,restriction fragment length polymorphisms,referrd as RFLPs,has been widely applied to the prenatal diagnosis of some genetic dise ases. 摘要 限制性酶切片段长度多态性(RFLP)作为共显性的遗传标记,已广泛应用于遗传病的产前诊 断。
- Methods Large restriction fragment (LRF) pattern analysis of genomic DNA by using pulsed-field gel electrophoresis was performed and differences of patterns were compared. 方法采用核酸脉冲电泳场分析技术,分析53株临床分离株酶切电泳图谱,并比较图谱的差异。
- Methods Large restriction fragment (LRF) pattern analysis of genomic DNA by using pulsed field gel electrophoresis was performed and differences of patterns were compared. 方法采用核酸脉冲电泳场分析技术,分析53株临床分离株酶切电泳图谱,并比较图谱的差异。
- The application of restriction fragment length polymophism(RFLP) of mitochondrial DNA(mtDNA) in the study of genetic diversity of domestic animals was summarized in this article. 对线粒体DNA限制性片段长度多态性(RFLP)在家养动物遗传多样性研究中的应用现状进行了综述,并对其应用前景进行了展望。
- In this study, a technique describing aquatic bacterial communitystructure has been developed by using restriction fragment length polymorphism of 16S ribosomal RNA gene (rDNA). 用细菌16S核糖体RNA基因(rDNA)限制性片段长度多型性描述了水环境细菌群落结构。
- Method Polymerase chain reaction and restriction fragment length polymorphism were used to examine 40 oral malignant tumors for loss of heterozygosity at chromosome 3p. 方法应用聚合酶链反应限制性片段长度多态性分析方法对40例口腔鳞癌组织中3号染色体的等位基因杂合缺失进行测定。
- Errors intrinsic to the testing systems,such as the inability to precisely measure DNA restriction fragment lengths,are well compensated for by interpretation guidelines which take these kinds of errors into account. 鉴定系统本身固有的误差,如不能精确的测量DNA限制酵素片段长度,可以由解释规则来很好的弥补,因为解释规则会考虑到这些种类的误差。
- It isconsisted of 115 simple sequence repeat (SSR) markers, 84 random amplified polymorphicDNA (RAPD), 47 restriction fragment length polymorphism (RFLP) markers, 7 isozymes, and 3morphological markers. 构建了一个具有256个遗传标记,分布较好的完整的分子连锁图谱,其中包括115个SSR标记、84个RAPD标记、47个RFLP标记、7个同功酶标记和3个形态标记。
- In the F 2 population from npa 1/Sheng47 the gene for the non palea trait was mapped between two restriction fragment length polymorphism markers, C498 and RZ450, with distances of 7.5cM and 2.4cM, respectively. 选用突变体为父本、生 4 7为母本杂交的F2 群体作定位群体 ,利用SSLP标记和RFLP标记将与突变性状相关的基因定位在第 6染色体短臂上RFLP标记C4 98和RZ4 5 0之间 ,暂定名为npa 1。
- Errors intrinsic to the testing systems, such as the inability to precisely measure DNA restriction fragment lengths, are well compensated for by interpretation guidelines which take these kinds of errors into account. 鉴定系统本身固有的误差,如不能精确的测量DNA限制酵素片段长度,可以由解释规则来很好的弥补,因为解释规则会考虑到这些种类的误差。
- Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). 方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
- Methods To examine the APOE genotypes and alleles in 58 AD patients, 10 VD patients and 96 healthy controls with PCR and restriction fragment length polymorphisms (RFLPs) technique. 方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法对58例无亲缘关系的中国昆明地区AD患者、10例VD患者及96例健康老年人,进行ApoE基因变异检测,并进行统计分析。
- Methods The polymerase chain reaction restriction fragment length polymorphism was used to detect VDR genotype in 40 patients with osteoporosis and 21 healthy postmenopausal women. 方法用聚合酶链反应限制性片段长度多态性技术检测了40例绝经后患骨质疏松症的妇女和21名同龄绝经后无骨质疏松症的妇女的维生素D受体基因多态性。
- Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) methods were used to measure DNA damage repair gene XPC and folate metabolism enzyme gene MTHFR polymorphisms. 对照组选自同期居住在扬中境内的居民,与病例按性别和年龄组进行成组频数匹配,要求身体健康,无恶性肿瘤及胃和十二指肠溃疡病史,征得同意后采集静脉血并进行流行病学问卷调查。
- Methods: APOE _219G/T genetype in AMD group(50 cases)and control group(95 persons)was identified by polymerase chain reaction and restriction fragment length polymorphism genotyping technique. :用聚合酶链反应_限制性片断长度多态性分析法对AMD组(50例)和对照组(95例)的APOE_219G/T基因型做出鉴定。