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- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应( R T? P C R),对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- effect of As ODN on ppET 1 mRNA expression of GMC was measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR); 用半定量反转录聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)检测寡核苷酸链对GMC的 ppET 1mRNA表达的影响 ;
- The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection. 收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Fluorescent Quantitative RT - PCR 荧光定量RT-PCR
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- It can give the qualitative, semi quantitative, or quantitative data about the compositions of fluid inclusions quickly. 它可以快速方便地对单个包裹体进行定性、半定量乃至定量分析,且样品制备简单。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- real - time quantitative RT - PCR 实时定量RT-PCR
- real-time fluorescence quantitative RT - PCR 实时荧光定量RT-PCR
- Real - time fluorescence quantitative RT - PCR 实时荧光定量PCR
- With semi quantitative noncompetitive RT-PCR assay, the hTERT mRNA expression in IMR90 cells was negative and the normalized hTERT mRNA expression in T24 cells was 0.06[hTERT(IOD)/GAPDH(IOD)]. 半定量非竞争RT-PCR检测IMR90和T24细胞株hTERT mRNA的表达,IMR90细胞株hTERT mRNA无表达,T24细胞株hTERT mRNA表达为0.;06[hTERT(IOD)/GAPDH(IOD)]。
- RT PCR for EWS FLI 1 is sensitive enough to detect minimal residual Ewing's sarcoma cells in the bone marrow. 以EWS FLI 1融合基因为标志的反向PCR测定骨髓Ewing肉瘤细胞的灵敏度为 10 -6,它是测定骨髓Ewing肉瘤细胞高度敏感的方法 ;
- The positive rate of MDR1 mRNA was 70.3% with improved RT PCR in 37 among 49 cases of ovarian carcinomas. 有37 例卵巢癌用改进的RT?PCR 检测MDR1 基因m RNA 表达的阳性率为70?3 %25 。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。
- Methods B7 expresion was studied by means of RT PCR and immunohistochemistry (IH) in 45 tumors of the urinary system. 方法应用逆转录?聚合酶链反应(RT?PCR)和免疫组织化学方法,检测45例泌尿系肿瘤B7基因在mRNA和蛋白水平的表达情况。