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- serotype specific primer 分型引物
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物,扩增出运动蛋白基因的整个开放阅读框(ORF)。
- A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E. 根据已知的cry2Ab基因ORF序列,设计合成了一对特异性引物,PCR扩增出cry2Ab基因全长,并直接与pUCm-T载体相连。
- Methods HLA-B* gene polymorphism in 31 patients with DCM and 29 normal control subjects was analyzed using polymerase chain reaction-sequence specific primer(PCR-SSP) technique. 方法采用聚合酶链反应和顺序特异性引物(PCR-SSP)基因多态性分析方法,对31例扩张型心肌病患者及29例无血缘关系健康人的HLA-B*各等位基因及亚基因进行检测分析。
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因(apxI,apxII,apxIII,apxIV)。
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同,需通过DGGE检测证明是一种DNA序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素(LT)的基因序列设计引物,用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
- Methods PCR-SSP was set up by synthesized 29 specific primers and 1 pairs of internal control primer,20 PCR reaction for DR alleles. 方法合成29个特异性引物和1对阳性对照引物,组成20个PCR反应用于DR位点,建立一步法PCR-SSP。
- specific primer on the mutated site 突变位点特异性引物
- Sequence specific primer ( PCR - SSP) 顺序特异性引物
- Polymerase chain reaction- sequence specific primer 聚合酶链反应-序列特异引物法
- polymerase chain reaction-sequence specific primer 聚合酶链反应-序列特异性引物
- The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. 采用PCR-序列特异性引物技术(SSP)对HLA-DQB作中分辨法分型。
- Methods Type specific primers and PCR were used to detect the HBV genotypes of 127 Uighur CHB patients in Xinjiang. 方法采用型特异性引物巢式PCR法对127例维吾尔族慢性乙型肝炎患者进行基因分型,并测序验证。
- Two pairs of specific primers: Tch old and Tch new primers were designed based on the result of 18S rRNA gene sequence. 经试验证明这两对引物能够非常有效的区分吕氏泰勒虫和尤氏泰勒虫。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Antje J B.Nicole A S.Naomi S S Biosensor for dengue virus detection:sensitive,rapid,and serotype specific 2002(6 王卓.;周真
- According to the four types of virulence genes: stx1, stx2, eaeA and hlyA, four pairs of specific primers have been designed. [方法]针对产志贺毒素大肠杆菌(STEC)的stx1、stx2、eaeA、hlyA4种毒力基因,设计了4对特异性引物。
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。