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- Designed and synthesised 4 fragments of antibacterial peptide B gene, then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing). 摘要人工设计并合成了抗菌肽B基因的4个寡聚核苷酸片段,通过重叠区扩增法,扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。
- Over the twenty years, it has branched into three characteristic strategies: splicing by overlapping extension (SOE), jumping polymerase chain reaction (JPCR) and DNA shuffling. 经过二十多年的发展,该技术已形成三个各具特色的方法分支:重叠延伸拼接、跳跃PCR和DNA重排。
- Gene splicing by overlap extension, SOE 作了改进,在拼接延伸后再进行一次
- splicing by overlap extension (SOE) 重叠延伸技术(SOE)
- splicing by overlap extension PCR 重叠延伸PCR(SOEPCR)
- Gene splicing by overlap extension (SOEing) 重叠区扩增基因拼接法(SOEing)
- gene splicing by overlap extension 重叠区扩增基因拼接法
- gene splicing by overlap extension PCR 重叠延伸PCR
- PCR splicing by overlap extension 重叠伸展的PCR缝接
- Keywords Th cell epitop;DNA vaccine;splicing by overlap extension; 辅助细胞表位;DNA疫苗;互补延伸拼接;
- hepacivirus,viral core proteins,mutation,eukaryotic expression, splicing by overlap extension(SOE 肝炎病毒;病毒核心蛋白质类;突变;真核表达;重叠延伸拼接法
- Keywords Staphylococcal enterotoxin A;Alkaline phosphatase;Gene splicing by overlap extension; 关键词金黄色葡萄球菌肠毒素A;碱性磷酸酶;重叠区扩增基因拼接法;
- splicing by overlap extension PCR重叠延伸技术
- A and B fragments of CYP2J3 gene were connected by overlap extension PCR to obtain ORF sequence of CYP2J3 gene. 运用重叠延伸聚合酶链反应连接CYP2J3基因A和B两片段,扩增出大鼠CYP2J3基因全长ORF序列。
- splicing proliferation by overlap extension PCR重叠延伸技术
- splicing by overlap extension-PCR 重叠延伸PCR
- By overlapping these fileds from two singularities. 从两个奇点处重叠这些重力场。
- Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T. 方法:应用Overlap PCR的方法制备Protein A的Z结构域(Protein A-Z),克隆于pMD-18T载体构建PA-Z/pMD-18T重组质粒。
- In order to improve the optimum temperature of lipases, The Penidilum expansuin lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. 利用重叠延伸PCR法对扩展青霉碱性脂肪酶(PEL)基因作了体外定点突变,获得了最适作用温度有所提高的突变体。
- He persuaded her to sign the document by guile. 他用欺骗手段说服她在文件上签字。